Active Recombinant Human GZMB Protein (19-247aa), C-His tagged

• Cat.No.: GZMB-33H
• Product Overview: Recombinant human GZMB (19-247aa), fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Description: This gene encodes a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis.
• Source: Baculovirus
• Species: Human
• Tag: His
• Form: Liquid
• Bio-activity: > 7, 000 pmol/min/μg, and is defined as the amount of enzyme that cleave 1pmole of Boc-Ala-Ala-Asp-SBzl at 37 centigrade.
• Molecular Mass: 26.5 kDa (235 aa)
• Protein length: 19-247 aa
• AA Sequence: GEIIGGHEAKPHSRPYMAYLMIWDQ KSLKRCGGFLIRDDFVLTAAHCWGS SINVTLGAHNIKEQEPTQQFIPVKR PIPHPAYNPKNFSNDIMLLQLERKA KRTRAVQPLRLPSNKAQVKPGQTCS VAGWGQTAPLGKHSHTLQEVKMTVQ EDRKCESDLRHYYDSTIELCVGDPE IKKTSFKGDSGGPLVCNKVAQGIVS YGRNNGMPPRACTKVSSFVHWIKKT MKRY< HHHHHH>
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 95 % by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/mL (determined by absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 20 % glycerol, 1mM DTT.

Gene Information

• Gene Name: GZMB granzyme B [ Homo sapiens (human) ]
• Official Symbol: GZMB
• Synonyms: GZMB; granzyme B; C11; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1; granzyme B; T-cell serine protease 1-3E; cathepsin G-like 1; cytotoxic T-lymphocyte proteinase 2; cytotoxic serine protease B; fragmentin 2; granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1); human lymphocyte protein; EC 3.4.21.79
• Gene ID: 3002
• mRNA Refseq: NM_004131
• Protein Refseq: NP_004122
• MIM: 123910
• UniProt ID: P10144

Reviews

11/01/2020

I am delighted to express my complete satisfaction with the quality of GZMB protein, as it precisely suits my experimental requirements.

08/29/2017

Their knowledgeable team has been readily available to address any concerns or queries, providing effective solutions and guidance whenever needed.

07/28/2017

I have full confidence in the GZMB protein and the exceptional support provided by the manufacturer, making it my top recommendation for researchers seeking a high-quality protein for their experimental needs.

Q&As

What are some potential future applications of GZMB in medicine?

GZMB may be used in the development of targeted therapies for viral infections and various immune-related disorders.

How is GZMB protein clinically relevant in cancer treatment?

GZMB can be used in cancer immunotherapy to target and eliminate cancer cells.

What are the challenges in using GZMB in cancer immunotherapy?

One challenge is to ensure that GZMB selectively targets cancer cells without causing harm to healthy cells.

What types of cancer can be targeted using GZMB-based therapies?

GZMB-based therapies can be used against various types of cancer, including leukemia, lymphoma, and solid tumors.

Can GZMB be used in autoimmune disease treatment?

GZMB's role in autoimmune diseases is complex. It can contribute to tissue damage in some autoimmune conditions, but its use in treatment is not common.