| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
Non |
| Protein Length : |
amino acids 2–1215 |
| Description : |
HDAC6 is a Class II HDAC. It is unique among human HDACs in having two full deacetylase domains. As a tubulin deacetylase, HDAC6 may act to promote autophagic clearing of Huntington aggregates and retard HIV1 infection. It can shuttle between the nucleus and cytoplasm, suggesting potential extra nuclear functions by regulating the acetylation status of non-histone substrates. By modifying chromatin structure and other non-histone proteins, HDACs play important roles in controlling complex biological events, including cell development, differentiation, programmed cell death, angiogenesis, and inflammation. Considering these major roles, it is conceivable that dysregulation of HDACs and subsequent imbalance of acetylation and deacetylation may be involved in the pathogenesis of various diseases, including cancer and inflammatory diseases. |
| Form : |
Liquid, In 30 mM HEPES, 140 mM NaCl, 10 mM KCl, 3% glycerol, 0.25 mM TCEP, pH 7.4. |
| Bio-activity : |
Deacetylation activity was determined using GAK(Ac)-AMC, Boc-K(Ac)-AMC and Fluor-de-Lys (RHK-K(Ac)-AMC) substrates. Reactions were carried out in a 384-well plate in 20 µL of reaction buffer comprising of 50 mM HEPES, 140 mM NaCl, 10 mM KCl, 1 mg/ml bovine serum albumin (BSA), and 1 mM TCEP, pH 7.4, for 30 min at 37°C. The reaction was stopped by addition of 20 µl of trypsin solution (2 mg/ml trypsin) and a fluorescence signal of released AMC was quantified using a fluorometer. The enzyme kinetic values were calculated by non-linear regression analysis. |
| Molecular Mass : |
131 kDa |
| Purity : |
≥90% by SDS-PAGE |
| Notes : |
For Research Use Only! Not For Use in Humans. |
| Storage : |
Store at -80 centigrade. |
| Handling : |
Thaw on ice, and aliquot into smaller working quantities to avoid multiple freeze/thaw cycles. |
