Recombinant Full Length Human HDAC6 Protein, C-Flag-tagged

• Cat.No.: HDAC6-255HFL
• Product Overview: Recombinant Full Length Human HDAC6 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 131.2 kDa
• AA Sequence: MTSTGQDSTTTRQRRSRQNPQSPPQ DSSVTSKRNIKKGAVPRSIPNLAEV KKKGKMKKLGQAMEEDLIVG LQGMDLNLEAEALAGTGLVLDEQLN EFHCLWDDSFPEGPERLHAIKEQLI QEGLLDRCVSFQARFAEKEE LMLVHSLEYIDLMETTQYMNEGELR VLADTYDSVYLHPNSYSCACLASGS VLRLVDAVLGAEIRNGMAII RPPGHHAQHSLMDGYCMFNHVAVAA RYAQQKHRIRRVLIVDWDVHHGQGT QFTFDQDPSVLYFSIHRYEQ GRFWPHLKASNWSTTGFGQGQGYTI NVPWNQVGMRDADYIAAFLHVLLPV ALEFQPQLVLVAAGFDALQG DPKGEMAATPAGFAQLTHLLMGLAG GKLILSLEGGYNLRALAEGVSASLH TLLGDPCPMLESPGAPCRSA QASVSCALEALEPFWEVLVRSTETV ERDNMEEDNVEESEEEGPWEPPVLP ILTWPVLQSRTGLVYDQNMM NHCNLWDSHHPEVPQRILRIMCRLE ELGLAGRCLTLTPRPATEAELLTCH SAEYVGHLRATEKMKTRELH RESSNFDSIYICPSTFACAQLATGA ACRLVEAVLSGEVLNGAAVVRPPGH HAEQDAACGFCFFNSVAVAA RHAQTISGHALRILIVDWDVHHGNG TQHMFEDDPSVLYVSLHRYDHGTFF PMGDEGASSQIGRAAGTGFT VNVAWNGPRMGDADYLAAWHRLVLP IAYEFNPELVLVSAGFDAARGDPLG GCQVSPEGYAHLTHLLMGLA SGRIILILEGGYNLTSISESMAACT RSLLGDPPPLLTLPRPPLSGALASI TETIQVHRRYWRSLRVMKVE DREGPSSSKLVTKKAPQPAKPRLAE RMTTREKKVLEAGMGKVTSASFGEE STPGQTNSETAVVALTQDQP SEAATGGATLAQTISEAAIGGAMLG QTTSEEAVGGATPDQTTSEETVGGA ILDQTTSEDAVGGATLGQTT SEEAVGGATLAQTTSEAAMEGATLD QTTSEEAPGGTELIQTPLASSTDHQ TPPTSPVQGTTPQISPSTLI GSLRTLELGSESQGASESQAPGEEN LLGEAAGGQDMADSMLMQGSRGLTD QAIFYAVTPLPWCPHLVAVC PIPAAGLDVTQPCGDCGTIQENWVC LSCYQVYCGRYINGHMLQHHGNSGH PLVLSYIDLSAWCYYCQAYV
  HHQALLDVKDIAHQNKFGEDMPHPH TRTRPLEQKLISEEDLAANDILDYK DDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Transcription Factors

Gene Information

• Gene Name: HDAC6 histone deacetylase 6 [ Homo sapiens (human) ]
• Official Symbol: HDAC6
• Synonyms: HD6; JM21; CPBHM; PPP1R90
• Gene ID: 10013
• mRNA Refseq: NM_006044.4
• Protein Refseq: NP_006035.2
• MIM: 300272
• UniProt ID: Q9UBN7