Active Recombinant Human IGFBP4 Protein, His-tagged

• Cat.No.: IGFBP4-184I
• Product Overview: Recombinant Human IGFBP4 Protein with His tag was expressed in HEK 293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Description: Insulin-like growth factor-binding protein 4 (IGF-BP-4), also known as IBP-4, is a secreted glycoprotein belonging to the IGFBP family. IGF-BP-4 is produced by osteoblasts, epidermis, ovarian follicles and other tissues. It binds both insulin-like growth factor (IGF) I and II, and it circulates in the plasma in both glycosylated and non-glycosylated forms. IGF-BP-4 prolongs the half-life of the IGFs and has been shown to inhibit or stimulate the growth-promoting effects of the IGFs. Pregnancy Associated Plasma Protein A (PAPP-A) proteolytically cleaves IGF-BP-4 and reduces its affinity to bind IGFs, and thus serves as an important regulator of IGF-BP-4 function.
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: ED50 < 50 ng/mL, measured in a bioassay using FDC-P1 cells in the presence of 15 ng/mL human IGF-II.
• Molecular Mass: 30-35 kDa, observed by reducing SDS-PAGE.
• AA Sequence: DEAIHCPPCSEEKLARCRPPVGCEELVREPGCGCCATCALGLGMPCGVYTPRCGSGLRCYPPRGVEKPLHTLMHGQGVCMELAEIEAIQESLQPSDKDEGDHPNNSFSPCSAHDRRCLQKHFAKIRDRSTSGGKMKVNGAPREDARPVPQGSCQSELHRALERLAASQSRTHEDLYIIPIPNCDRNGNFHPKQCHPALDGQRGKCWCVDRKTGVKLPGGLEPKGELDCHQLADSFREHHHHHH
• Endotoxin: < 0.2 EU/μg, determined by LAL method.
• Purity: > 95% as analyzed by SDS-PAGE.
• Storage: Lyophilized recombinant human IGF-BP-4 remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, human IGF-BP-4, His should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade.
• Storage Buffer: Lyophilized after extensive dialysis against PBS.
• Reconstitution: Reconstituted in ddH2O or PBS at 100 μg/mL.

Gene Information

• Gene Name: IGFBP4 insulin-like growth factor binding protein 4 [ Homo sapiens ]
• Official Symbol: IGFBP4
• Synonyms: IGFBP4; insulin-like growth factor binding protein 4; insulin like growth factor binding protein 4; insulin-like growth factor-binding protein 4; BP 4; HT29 IGFBP; IBP4; IGF binding protein 4; IGFBP 4; IBP-4; IGF-binding protein 4; BP-4; IGFBP-4; HT29-IGFBP
• Gene ID: 3487
• mRNA Refseq: NM_001552
• Protein Refseq: NP_001543
• MIM: 146733
• UniProt ID: P22692