| Description : |
Insulin-like growth factor-binding protein 4 (IGF-BP-4), also known as IBP-4, is a secreted glycoprotein belonging to the IGFBP family. IGF-BP-4 is produced by osteoblasts, epidermis, ovarian follicles and other tissues. It binds both insulin-like growth factor (IGF) I and II, and it circulates in the plasma in both glycosylated and non-glycosylated forms. IGF-BP-4 prolongs the half-life of the IGFs and has been shown to inhibit or stimulate the growth-promoting effects of the IGFs. Pregnancy Associated Plasma Protein A (PAPP-A) proteolytically cleaves IGF-BP-4 and reduces its affinity to bind IGFs, and thus serves as an important regulator of IGF-BP-4 function. |
| Source : |
HEK 293 |
| Species : |
Human |
| Tag : |
His |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
ED50 < 50 ng/mL, measured in a bioassay using FDC-P1 cells in the presence of 15 ng/mL human IGF-II. |
| Molecular Mass : |
30-35 kDa, observed by reducing SDS-PAGE. |
| AA Sequence : |
DEAIHCPPCSEEKLARCRPPVGCEE LVREPGCGCCATCALGLGMPCGVYT PRCGSGLRCYPPRGVEKPLHTLMHG QGVCMELAEIEAIQESLQPSDKDEG DHPNNSFSPCSAHDRRCLQKHFAKI RDRSTSGGKMKVNGAPREDARPVPQ GSCQSELHRALERLAASQSRTHEDL YIIPIPNCDRNGNFHPKQCHPALDG QRGKCWCVDRKTGVKLPGGLEPKGE LDCHQLADSFREHHHHHH |
| Endotoxin : |
< 0.2 EU/μg, determined by LAL method. |
| Purity : |
> 95% as analyzed by SDS-PAGE. |
| Storage : |
Lyophilized recombinant human IGF-BP-4 remains stable up to 6 months at -80 centigrade from date of receipt. Upon reconstitution, human IGF-BP-4, His should be stable up to 1 week at 4 centigrade or up to 2 months at -20 centigrade. |
| Storage Buffer : |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution : |
Reconstituted in ddH2O or PBS at 100 μg/mL. |
