Recombinant Human IGFBP4 protein
Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.
|Product Overview :||Recombinant Human IGFBP4 (Glu99-Lys327) protein carried no tag and was expressed in human 293 cells (HEK293).|
|Description :||Immunoglobulin G4 (IgG4) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. Ig gamma-4 chain Fc region contains two constant regions of IgG4 H chain (CH2, CH3).|
|Predicted N Terminal :||Glu99|
|Form :||Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 25.8 kDa. As a result of glycosylation, the protein migrates as 30-32 kDa under reducing (R) condition, and 28-30 kDa and 55-60 kDa under non-reducing (NR) condition (SDS-PAGE).|
|Protein length :||Glu99-Lys327|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.|
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of IGFBP4-755H :||
Human IgG4 Fc, Tag Free on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
|Bioactivity-SPR of IGFBP4-755H :||
Human FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI verified) captured on CM5 Chip via anti-His antibody can bind Human IgG4 Fc, Tag Free (SPR verified) with an affinity constant of 0.715 μM as determined in SPR assay (Biacore 8K) (QC tested).
|Gene Name :||IGFBP4|
|Official Symbol :||IGFBP4|
|Synonyms :||IGFBP4; insulin-like growth factor binding protein 4; insulin like growth factor binding protein 4; insulin-like growth factor-binding protein 4; BP 4; HT29 IGFBP; IBP4; IGF binding protein 4; IGFBP 4; IBP-4; IGF-binding protein 4; BP-4; IGFBP-4; HT29-IGFBP|
|Gene ID :||3487|
|mRNA Refseq :||NM_001552|
|Protein Refseq :||NP_001543|
|UniProt ID :||P22692|
◆ Recombinant Protein
|IGFBP4-1389H||Recombinant Human IGFBP4 Protein, MYC/DDK-tagged||+Inquiry|
|IGFBP4-1385H||Recombinant Human IGFBP4 Protein||+Inquiry|
|Igfbp4-1415R||Recombinant Rat Insulin-Like Growth Factor Binding Protein 4||+Inquiry|
|IGFBP4-834P||Recombinant Pig IGFBP4 protein, His & T7-tagged||+Inquiry|
|Igfbp4-833M||Recombinant Mouse Igfbp4 protein, His & T7-tagged||+Inquiry|
|IGFBP4-1231CCL||Recombinant Cynomolgus IGFBP4 cell lysate||+Inquiry|
|IGFBP4-2926HCL||Recombinant Human IGFBP4 cell lysate||+Inquiry|
|IGFBP4-2925MCL||Recombinant Mouse IGFBP4 cell lysate||+Inquiry|