Active Recombinant Human IL10RB, MIgG2a Fc-tagged

Cat.No. : IL10RB-201H
Product Overview : The extracellular domain of human IL-10 (NP_000563.1)(Ser19-Lys178) (Glu151Lys) is fused to the N-terminus of the Fc region of mouse IgG2a was expressed in CHO cell.
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Description : The protein encoded by this gene belongs to the cytokine receptor family. It is an accessory chain essential for the active interleukin 10 receptor complex. Coexpression of this and IL10RA proteins has been shown to be required for IL10-induced signal transduction. This gene and three other interferon receptor genes, IFAR2, IFNAR1, and IFNGR2, form a class II cytokine receptor gene cluster located in a small region on chromosome 21
Source : CHO cell
Species : Human
Tag : MIgG2a Fc-tagged
Form : Lyophilized from 0.2μm-filtered solution in PBS.
Bio-activity : Bioactivity demonstrated in cytokine synthesis inhibition assay, measuring inhibition of IL-6 production by PU5-1 cells.
Molecular Mass : 45 KDa (monomer)
AA Sequence : Glu151Lys
Endotoxin : <0.06 eu/μg as determined by lal test.>
Purity : >98%, by SDS-PAGE under reducing conditions.
Stability : Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Reconstitution : Reconstitute at 100μg/ml in sterile PBS.
Warning : Avoid freeze/thaw cycles.
Gene Name : IL10RB interleukin 10 receptor, beta [ Homo sapiens ]
Official Symbol : IL10RB
Synonyms : IL10RB; interleukin 10 receptor, beta; CRFB4, D21S58, D21S66; interleukin-10 receptor subunit beta; CDW210B; CRF2 4; IL 10R2; IL-10RB; IL-10R subunit 2; IL-10R subunit beta; IL-10 receptor subunit beta; cytokine receptor class-II CRF2-4; interleukin-10 receptor subunit 2; cytokine receptor class-II member 4; cytokine receptor family 2 member 4; cytokine receptor family II, member 4; CRFB4; CRF2-4; D21S58; D21S66; IL-10R2;
Gene ID : 3588
mRNA Refseq : NM_000628
Protein Refseq : NP_000619
MIM : 123889
UniProt ID : Q08334
Chromosome Location : 21q22.11
Pathway : Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Jak-STAT signaling pathway, organism-specific biosystem; Jak-STAT signaling pathway, conserved biosystem; Toxoplasmosis, organism-specific biosystem; Toxoplasmosis, conserved biosystem; Tuberculosis, organism-specific biosystem;
Function : contributes_to interleukin-10 receptor activity; protein binding; receptor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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Can you explain the role of mass spectrometry-based interactomics in identifying IL10RB-interacting proteins? 12/17/2022

Mass spectrometry-based interactomics identifies proteins interacting with IL10RB.

How does NMR spectroscopy help in understanding dynamic interactions and structural changes in IL10RB upon ligand binding? 11/29/2022

NMR spectroscopy helps understand dynamic interactions and structural shifts in IL10RB upon binding.

How do proximity labeling and quantitative proteomics reveal changes in IL10RB's protein network upon IL-10 stimulation? 02/12/2022

Proximity labeling and quantitative proteomics show IL10RB's changing protein network with IL-10.

How do techniques like surface plasmon resonance and isothermal titration calorimetry contribute to studying IL10RB-IL-10 binding kinetics? 11/09/2020

Techniques like surface plasmon resonance and isothermal titration calorimetry measure IL10RB-IL-10 binding strength and kinetics.

What insights do flow cytometry and single-cell transcriptomics provide about IL10RB's expression across cell types and immune subsets? 03/22/2020

Flow cytometry and single-cell transcriptomics reveal IL10RB's expression diversity in cells and immune subsets.

Could you explain how mutagenesis and alanine scanning identify critical amino acids in IL10RB's binding to IL-10? 05/11/2019

Mutagenesis and alanine scanning pinpoint amino acids essential for IL10RB's binding to IL-10.

How are CRISPR-edited cell lines and gene knockout models utilized to explore IL10RB's downstream signaling pathways after IL-10 binding? 03/18/2019

CRISPR-edited cells and knockout models explore IL10RB's signaling after IL-10 binding.

What methods are used to reveal structural changes in IL10RB upon interaction with IL-10, such as X-ray crystallography and cryo-electron microscopy? 10/21/2018

X-ray crystallography and cryo-electron microscopy provide structural insights into IL10RB's changes upon IL-10 interaction.

What does genome-wide CRISPR screening reveal about genes and pathways influencing IL10RB expression and function? 05/03/2018

Genome-wide CRISPR screening uncovers genes affecting IL10RB expression and function.

What experimental approaches, like co-immunoprecipitation and FRET, are used to study protein complexes involving IL10RB and IL-10? 04/14/2017

Co-immunoprecipitation and FRET study protein complexes involving IL10RB and IL-10.

Customer Reviews (3)

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Reviews
04/28/2022

    Reliable in co-immunoprecipitation.

    06/13/2020

      High specificity.

      12/04/2019

        Effective in signaling pathways.

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