Species : |
Human |
Source : |
Yeast |
Tag : |
Non |
Protein Length : |
133 a.a. |
Description : |
Human IL-2 (also known as TCGF) is an about 15.4KD factor produced mainly by activated CD4+ T cells. IL-2 induces cell cycle progression of resting cells in an antigen non-specific manner and allows clonal expansion of activated T cells. IL-2 also acts on activated B cells, monocytes, NK, LAK cells, and on oligodendroglial cells in vitro. In addition, IL-2 plays a role in hematopoiesis, tumor surveillance and anti-inflammatory reactions and hence is a central regulator of the immune response. Non-glycosylated IL-2 is biologically active. Recombinant human IL-2 is biologically active and can promote proliferation of T lymphocytes in culture. |
Form : |
Lyophilized from a 0.2μm filtered solution in 10mM Phosphate buffer containing 0.3% human serum albumin. |
Bio-activity : |
Measured in a cell proliferation assay using an IL-2 dependent Mouse cytotoxic T cell line, CTLL-2. The specific activity shall be not less than 3×10^7 IU/mg. |
Endotoxin : |
≤1EU/μg, determined by the LAL method |
Purity : |
≥97%, as determined by SDS-PAGE and HPLC method. |
Usage : |
FOR RESEARCH ONLY |
Storage : |
Lyophilized samples are stable for greater than six months from date of receipt at -20 centigrade to -70 centigrade. The reconstituted samples can be stored under sterile conditions at 2-8 centigrade for one month or at -20 centigrade to -70 centigrade for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile ddH2O containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial, to prepare a stock solution of not less than 1μg/ml. |