Active Recombinant Human IL2 Protein

• Cat.No.: IL2-98H
• Product Overview: Recombinant Human Interleukin-2 is produced by our E. coli expression system and the target gene encoding Ala21-Thr153 is expressed.

Specification

• Description: Recombinant Human Interleukin-2 is a highly purified protein with a molecular weight of approximately 15,300 Daltons. The chemical name is des-alanyl-1, serine-125 Human Interleukin-2. It is produced by recombinant DNA technology using a genetically engineered E. coli strain containing an analog of the human interleukin-2 gene. Genetic engineering techniques were used to modify the Human IL-2 gene, and the resulting expression clone encodes a modified Human IL-2. This recombinant form differs from native Interleukin-2 in following ways: 1) it is not glycosylated; 2) the molecule has no N-terminal alanine; 3) the molecule has serine substituted for cysteine at amino acid position 125; 4) the aggregation state of molecule is likely to be different from that of native IL-2.
• Source: E. coli
• Species: Human
• Form: Lyophilized from a 0.2 um filtered solution of 20mM HAc-NaAc, 150mM NaCl, pH 4.0.
• Bio-activity: Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The specific activity of Recombinant Human IL-2 is ≥1×107 IU/mg
• Molecular Mass: Predicted Molecular Weight: 15.5kDa
  Apparent Molecular Weight: 14kDa, reducing conditions.
• AA Sequence: Pro22-Thr153
• Endotoxin: Less than 0.1 ng/ug (1 EU/ug).
• Purity: >95%
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days.
  Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months.
• Quality Statement: Purity Greater than 95% as determined by reducing SDS-PAGE
• Shipping: The product is shipped at ambient temperature.
  Upon receipt, store it immediately at the temperature listed below.

Gene Information

• Gene Name: IL2 interleukin 2 [ Homo sapiens (human) ]
• Official Symbol: IL2
• Synonyms: Interleukin-2; IL-2; T-Cell Growth Factor; TCGF; Aldesleukin; lymphokine
• Gene ID: 3558
• mRNA Refseq: NM_000586.4
• Protein Refseq: NP_000577.2
• MIM: 147680
• UniProt ID: P60568