Active Recombinant Human IL2RB and IL2RG Protein, His&Avi tagged

• Cat.No.: IL2RB-74H
• Product Overview: IL-2Rβ/γ protein heterodimer contains IL-2Rβ and IL-2Rγ extracellular domains (Uniprot# P14784 and Uniprot# P31785) fused with a proprietary dimer motif followed by a His tag and an Avi tag at the IL-2Rβ C-terminus and IL-2Rγ C-terminus, respectively.

Specification

• Species: Human
• Source: HEK293T
• Tag: Avi&His
• Protein Length: IL2RB: 27-240 aa IL2RG: 23-262 aa
• Description: Human interleukin-2 beta/gamma receptor (IL-2Rβ/γ) is a member of the type I cytokine receptor family. IL-2Rβ/γ is also known as interleukin-2 β/γ receptor, IL-2RB/IL-2RG, and Cluster of Differentiation 25 (CD25). IL-2 receptor (IL-2R) can be composed of several combinations of three proteins or "chains": IL-2 receptor alpha (IL-2Rα), IL-2 receptor beta (IL-2Rβ), and IL-2 receptor gamma (IL-2Rγ). This IL-2Rβ/γ protein heterodimer is composed of IL-2Rβ and IL-2Rγ subunits to form the IL-2Rβ/γ heterodimer. On the cell membrane, the beta chain is complexed with Janus kinase 1 (JAK1) and the gamma chain complexes with Janus kinase 3 (JAK3); through these interactions, upon Interleukin-2 (IL-2) binding, intracellular signaling pathways are initiated. Memory-phenotype CD8+ CD44+ T cells and NK cells highly express the IL-2Rβ/γ form of the receptor. Stimulation of IL-2Rβ/γ has shown promise as an antitumor immunity booster and a recombinant protein mimicking the IL-2Rβ/γ heterodimer conformation can be crucial for therapeutic discovery.
• Molecular Mass: 69 kDa
• Homodimer/Heterodimer: Heterodimer
• Purity: Greater than 90% dimer form as determined by SDS-PAGE under non-reducing condition
• Application: Verified Applications: ELISA for IL-2Rβ-specific and IL-2Rγ-specific antibodies and Interleukin-2 (IL-2) ligand protein binding assays. Suggested Applications: SPR & BLI for IL-2Rβ-specific and IL-2Rγ-specific antibodies and IL-2 protein binding assays. Animal immunization, RUO.
• Storage: At -80 centigrade
• Storage Buffer: 0.2μm filtered PBS, pH 7.4, no preservatives
• Shipping: Frozen Dry Ice

Gene Information

• Gene ID: 3560
• Gene Name: IL2RB interleukin 2 receptor, beta [Homo sapiens (human)]
• Official Symbol: 3560
• Synonyms: IL2RB; interleukin 2 receptor, beta; IL15RB, interleukin 15 receptor, beta; interleukin-2 receptor subunit beta; CD122; p75; IL-2RB; CD122 antigen; IL-2R subunit beta; IL-2 receptor subunit beta; interleukin 15 receptor, beta; high affinity IL-2 receptor beta subunit; high affinity IL-2 receptor subunit beta; IL15RB; P70-75
• mRNA Refseq: NM_000878
• Official Symbol 2: IL2RB
• Gene ID 2: 3560
• Gene Name 2: IL2RB interleukin 2 receptor, beta [Homo sapiens (human)]
• mRNA Refseq 2: NM_000878
• Protein Refseq 2: NP_000869
• MIM 2: 146710
• UniProt ID 2: P14784