Active Recombinant Human IL2RB protein, His/Avi-tagged, Biotinylated IL2RB-051H

Active Recombinant Human IL2RB protein, His/Avi-tagged, Biotinylated

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Cat.No. : IL2RB-051H Optional Service: Optional requirements on this protein
Product Overview : Biotinylated Recombinant Human IL2RB(Ala27-Asp239) protein, fused to His/Avi tag at the C-terminus, was expressed in HEK293 cells .
Description : Functional IL-2 receptors can exist in two affinity states on cell surfaces, the high affinity complex consisting of heterotrimers of the alpha, beta, and gamma chains, and the intermediate affinity complex comprising heterodimers of the beta and gamma chains. Individual beta chains and alpha chains exhibit low affinity IL-2 binding and the gamma chain alone does not bind IL-2. In addition to their involvement in IL-2 mediated signal transduction, both the beta chain and gamma chain have been shown to be required for IL-15 mediated signaling. IL-2 R beta is a member of the cytokine receptor superfamily. Human IL-2 R beta cDNA encodes a 551 amino acid residue precursor Type I membrane protein with a 26 residue signal peptide, a 214 residue extracellular region, a 25 residue transmembrane region and a 286 residue cytoplasmic domain. A soluble IL-2 R beta (IL-2 sR beta ) has been identified in the culture supernatants of a human lymphoid cell line, YT, that displays IL-2 R beta. At present, the function of IL-2 sR beta is unclear. Recombinant human IL-2 sR beta binds IL-2 with low affinity and is not an effective IL-2 antagonist on cells displaying the high or intermediate affinity IL-2 signaling receptors. Nevertheless, IL-2 sR beta binds IL-15 with sufficient affinity to neutralize IL-15 biological activities. Our Avi-tag Biotinylated Recombinant Human IL‑2 R beta features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.
Source : HEK293 cells
Species : Human
Tag : His/Avi
Predicted N Terminal : Ala27
Form : Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Bio-activity : The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Biotinylated Recombinant Human IL2B His-tag Avi-tag is immobilized at 0.25 µg/mL (100 µL/well).
Molecular Mass : 36-45 kDa, under reducing conditions
Protein length : Ala27-Asp239
Endotoxin : <0.10 EU per 1 μg of the protein by the LAL method.
Purity : >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Applications : Bioactivity
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution : Reconstitute at 500 μg/mL in PBS.
SDS-PAGE :

2 μg/lane Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining.

Binding Activity :

Gene Name : IL2RB interleukin 2 receptor, beta [ Homo sapiens ]
Official Symbol : IL2RB
Synonyms : IL2RB; interleukin 2 receptor, beta; IL15RB, interleukin 15 receptor, beta; interleukin-2 receptor subunit beta; CD122; p75; IL-2RB; CD122 antigen; IL-2R subunit beta; IL-2 receptor subunit beta; interleukin 15 receptor, beta; high affinity IL-2 receptor beta subunit; high affinity IL-2 receptor subunit beta; IL15RB; P70-75;
Gene ID : 3560
mRNA Refseq : NM_000878
Protein Refseq : NP_000869
MIM : 146710
UniProt ID : P14784
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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