Species : |
Human |
Source : |
E.coli |
Tag : |
His |
Protein Length : |
Met1~Ile323 |
Tag : |
N-His |
Form : |
PBS, pH7.4, containing 0.01% SKL, 5% Trehalose. |
Bio-activity : |
Galectin-4(GAL4) is a protein that in humans is encoded by the LGALS4 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS4 is an S-type lectin that is strongly underexpressed in colorectal cancer. In this case, we chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant human GAL4 with 0.9% sodium chloride injection, add 50μL a serial dilution of GAL4 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control without GAL4, replace with 50μL 0.9% sodium chloride injection. Then add 50μL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The minimal effective concentration of GAL4 is 3.187μg/mL. |
Molecular Mass : |
37.2kDa |
Endotoxin : |
<1.0EU per 1μg (determined by the LAL method) |
Purity : |
> 92% |
Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
Reconstitution : |
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |