Active Recombinant Human POLR2A, CT Domain, His-tagged

• Cat.No.: POLR2A-2513H
• Product Overview: Recombinant human His tagged CTD is isolated from anE. colistrain that carries the coding sequence of human RNA pol II carboxy-terminal domain under the control of a T7 promoter. 44 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: The carboxy-terminal repeat domain (CTD) of the largest subunit of RNA pol II contains tandem repeats of a heptapeptide sequence Tyr-Ser-Pro-Thr-Ser-Pro-Ser which is highly conserved among eukaryotic organisms. There are two forms of RNA pol II in vivo, designated IIO, which is extensively phosphorylated at the CTD, and IIA, which is not phosphorylated. The IIA form preferentially enters the pre-initiation complex (PIC), whereas IIO is found in the elongating complex. The kinase activity of TFIIH can mediate CTD phosphorylation, although other kinases, including Cdc2, Ctk1, the Srb10-Srb11 kinase-cyclin pair, and P-TEFb, have also been implicated in CTD phosphorylation. A phosphatase responsible for the dephosphorylation of the CTD has also been identified. CTD phosphatase activity is regulated by TFIIB and TFIIF.The CTD has also been implicated in pre-mRNA processing, most likely functioning as a platform for the recruitment and assembly of factors involved in pre-mRNA processing.
• Applications: CTD has been applied to in vitro transcription assays, splicing assays and protein-protein interactions assays.
• Activity: 1 unit equals 1 nanogram of purified protein. 20 units are sufficient for reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay.
• Quality Control: The purified protein is greater than 95% homogenous based on SDS-PAGE gel analysis.
• Reagents Supplied: 1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 0.2 mM EDTA and 1mM DTT.
• Storage Conditions: Store at -80°C.
• Pathways: Huntington"s disease; Metabolic pathways; Purine metabolism; Purine metabolism; RNA polymerase; DNA Repair; Gene Expression; Influenza Infection; Regulatory RNA pathways; Transcription; mRNA Processing

Gene Information

• Gene Name: POLR2A polymerase (RNA) II (DNA directed) polypeptide A, 220kDa [ Homo sapiens ]
• Synonyms: POLR2A; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220; MGC75453; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II A; RNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; EC 2.7.7.48; EC 2.7.7.6
• Gene ID: 5430
• mRNA Refseq: NM_000937
• Protein Refseq: NP_000928
• MIM: 180660
• UniProt ID: P24928
• Chromosome Location: 17p13.1
• Function: DNA binding; DNA-directed RNA polymerase activity; RNA-directed RNA polymerase activity; magnesium ion binding; nucleotidyltransferase activity; protein binding; transferase activity; ubiquitin protein ligase binding; zinc ion binding