Active Recombinant Mouse Cx3cl1 Protein

• Cat.No.: Cx3cl1-2385M
• Product Overview: Purified recombinant protein of Mouse chemokine (C-X3-C motif) ligand 1 (Cx3cl1 / Fractalkine) without tag was expressed in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Protein Length: Leu22-Lys105
• Description: Acts as a ligand for both CX3CR1 and integrins. Binds to CX3CR1 and to integrins ITGAV:ITGB3 and ITGA4:ITGB1. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1. The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium.
• Bio-activity: Bioactivity was measured by its property to chemoattract BaF3-mCX3CR1 transfectants in a dose dependent manner.
• Molecular Mass: 9.59 kDa
• Endotoxin: Less than 0.01 ng/μg protein as determined by the LAL method
• Purity: > 98%, as determined by Coomassie stained SDS-PAGE.
• Stability: Unopened vial can be stored between 2 and 8 centigrade for up to 2 weeks, at -20 centigrade for up to 6 months, or at -70 centigrade or below until the expiration date. Aliquots can be stored between 2 and 8 centigrade for up to one week and stored at -20 centigrade or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: Protein formulated and vialed at >50 μg/mL in specified buffer
• Storage Buffer: 10 mM NaH2PO4, 0.15 M NaCl, pH 7.2

Gene Information

• Gene Name: Cx3cl1 chemokine (C-X3-C motif) ligand 1 [ Mus musculus (house mouse) ]
• Official Symbol: Cx3cl1
• Synonyms: CX3CL1; chemokine (C-X3-C motif) ligand 1; fractalkine; neurotactin; C-X3-C motif chemokine 1; small-inducible cytokine D1; CX3C membrane-anchored chemokine; small inducible cytokine subfamily D, 1; CX3C; Cxc3; Scyd1; ABCD-3; AB030188; AI848747; D8Bwg0439e
• Gene ID: 20312
• mRNA Refseq: NM_009142
• Protein Refseq: NP_033168
• UniProt ID: O35188