Active Human CX3CL1, Biotin-tagged

• Cat.No.: CX3CL1-103H
• Product Overview: It is a synthetic Protein with Biotin tag.

Specification

• Description: Fractalkine, also termed neurotactin, is a membrane-bound CX3C chemokine. The mature protein is part of a 397-amino acid precursor consisting of a chemokine domain (76 amino acids), a mucin stalk of 241 residues, a putative transmembrane domain (18 amino acids), and an intracellular tail of 37 amino acids. Within the chemokine domain the first two cysteine residues are separated by 3 amino acids. Fractalkine message is found at high concentrations in the brain, and also in kidney, lung and heart. Fractalkine is chemotactic for monocytes and may play a role in brain inflammation. Product provided is the 76 amino acid chemokine domain with a C-terminal biotin.
• Species: Human
• Tag: Biotin
• Form: Lyophilized
• Bio-activity: Activity determined by a chemotaxis assay using human monocytes. Chemotaxis was achieved using a concentration of 10ng/ml. Optimal concentration should be evaluated by serial dilutions.
• Applications: Activity Assay
• Storage: Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
• Reconstitution: Recommended reconstitution in sterile phosphate buffered saline which contains at least 0.1% human or bovine serum albumin to prepare a stocksolution of no less than 20 μg/ml and vortex thoroughly.

Gene Information

• Gene Name: CX3CL1 chemokine (C-X3-C motif) ligand 1 [ Homo sapiens ]
• Official Symbol: CX3CL1
• Synonyms: CX3CL1; chemokine (C-X3-C motif) ligand 1; SCYD1, small inducible cytokine subfamily D (Cys X3 Cys), member 1 (fractalkine, neurotactin); fractalkine; ABCD 3; C3Xkine; CXC3; CXC3C; neurotactin; NTN; C-X3-C motif chemokine 1; small-inducible cytokine D1; CX3C membrane-anchored chemokine; small inducible cytokine subfamily D (Cys-X3-Cys), member-1; small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin); NTT; SCYD1; ABCD-3
• Gene ID: 6376
• mRNA Refseq: NM_002996
• Protein Refseq: NP_002987
• MIM: 601880
• UniProt ID: P78423
• Chromosome Location: 16q13
• Pathway: Chemokine receptors bind chemokines, organism-specific biosystem; Chemokine signaling pathway, organism-specific biosystem; Chemokine signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Direct p53 effectors, organism-specific biosystem
• Function: chemokine activity; protein binding; receptor binding