Active Recombinant Mouse Huntingtin Interacting Protein 1, His-tagged

• Cat.No.: Hip1-5638M
• Product Overview: RecombinantMouse Hip1 protein was expressed in Murine myeloma cell line, with theMouse Hip Lys24-Arg678 & Asp52-Arg678 (N-terminus) and aC-terminal 10-His tag.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: His
• Protein Length: 24-678 a.a.
• Description: Hedgehogsignaling proteins act as mitogens, morphogens, or inducing factors in manydifferent cell types during embryonic development. They aid in growth, patterning,and morphogenesis in both vertebrates and insects. Hip1 (Hedgehog-interactingprotein) is a type I transmembrane protein identified for its ability to bindbiologically active Sonic Hedgehog. It is comprised of 700 aa, and includes ahydrophobic signal sequence, two EGF-like domains near the C-terminus, and a22 amino acid membrane-spanning region at the C-terminal end. Hip1 has only beenidentified in vertebrates and binds all three mammalian Hedgehogs: sonic(Shh),desert (Dhh), and Indian (Ihh).
• Form: Lyophilized from a0.2 um filtered solution in PBS with BSA as a carrier protein.
• N-terminalSequence: Lys24 & Asp52
• MolecularWeight: 76 kDa & 73 kDa
• Activity: Measuredby its ability to inhibit Sonic Hedgehog (Shh) induction of alkalinephosphatase production in C3H10T1/2 mouse fibroblasts. The ED50 for thiseffect is typically 1.5-7.5 μg/mL in the presence of 5 μg/mL rmShh.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 80-90kDa and 58-64 kDa, reducing conditions.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100μg/mL in sterile PBS
• Storage: Use a manual defrostfreezer and avoid repeated freeze-thaw cycles. 12 months from dateof receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.

Gene Information

• Gene Name: Hip1 huntingtin interactingprotein 1 [ Mus musculus ]
• Official Symbol: Hip1
• Synonyms: Hip1; huntingtininteracting protein 1; mKIAA4113;2610109B09Rik; A930014B11Rik; E130315I21Rik; KIAA4113; MGC27616; HIP-I; huntingtin-interactingprotein I
• Gene ID: 215114
• mRNA Refseq: NM_146001
• Protein Refseq: NP_666113
• UniProt ID: Q8C303
• Chromosome Location: 5
• Pathway: EGFR1 SignalingPathway; Huntington"s disease
• Function: actin binding; binding;clathrin binding; phosphatidylinositol binding; phospholipid binding; proteinbinding