Active Recombinant Mouse Ifng Protein

• Cat.No.: Ifng-077M
• Product Overview: Purified recombinant protein of Mouse interferon gamma (Ifng) (His23-Cys155) without tag was expressed in E.coli.

Specification

• Species: Mouse
• Source: E.coli
• Protein Length: 23-155 a.a.
• Description: This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mice deficient in this gene have increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases.
• Bio-activity: The ED50 is 0.2-0.6 ng/ml, corresponding to a specific activity of 5 - 1.67 x 10^6 units/mg, determined by inhibition of WEHI-279 cell proliferation induced by mouse IFNγ.
• Molecular Mass: 15.7 kDa
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method
• Purity: >98%, as determined by Coomassie stained SDS-PAGE.
• Stability: Unopened vial can be stored between 2 centigrade and 8 centigrade for up to 2 weeks, at -20 centigrade for up to 6 months, or at -70 centigrade or below until the expiration date. Aliquots can be stored between 2 centigrade and 8 centigrade for up to one week and stored at -20 centigrade or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: Protein formulated and vialed at >50 ug/mL in specified buffer
• Storage Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM TCEP; pH7.2

Gene Information

• Gene Name: Ifng interferon gamma [ Mus musculus (house mouse) ]
• Official Symbol: Ifng
• Synonyms: Ifng; interferon gamma; Ifg; IFN-g; interferon gamma; IFN-gamma; gamma interferon
• Gene ID: 15978
• mRNA Refseq: NM_008337
• Protein Refseq: NP_032363
• UniProt ID: P01580