Product overview : |
Recombinant Human IFNG (P01579) aa. (Gln24~Gln166) fused with His tag was produced in HEK293F cells. |
Description : |
This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. |
Source : |
HEK293F |
Species : |
Human |
Tag : |
His |
Form : |
Freeze-dried powder |
Molecular Mass : |
Predicted Molecular Mass: 18.4kDa; Accurate Molecular Mass: 22&25kDa as determined by SDS-PAGE reducing conditions. |
Protein length : |
Gln24~Gln166 |
Endotoxin : |
<1.0EU per 1ug (determined by the LAL method) |
Purity : |
> 95% |
Characteristic : |
Isoelectric point is 9.7. |
Applications : |
SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Reporter Assays; Purification; Amine Reactive Labeling |
Stability : |
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
Storage : |
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
Concentration : |
200ug/mL |
Storage buffer : |
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5% Trehalose and Proclin300. |
Reconstitution : |
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex |