| Description : |
IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNFα. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Human IL-10 active on murine cells, but murine IL-10 is inactive on human cells. |
| Source : |
E. coli |
| Species : |
Mouse |
| Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
| Bio-activity : |
Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent co-stimulation (with IL-4) of the proliferation of murine MC/9 cells was found to be < 2 ng/mL, corresponding to a specific activity of > 5 × 10^5 units/mg. |
| Molecular Mass : |
Approximately 18.7 kDa, a single non-glycosylated polypeptide chain containing 161 amino acids |
| Protein Length : |
161 |
| AA Sequence : |
MSRGQYSREDNNCTHFPVGQSHMLL ELRTAFSQVKTFFQTKDQLDNILLT DSLMQDFKGYLGCQALSEMIQFYLV EVMPQAEKHGPEIKEHLNSLGQKLK TLRMRLRRCHRFLPCENKSKAVEQV KSDFNKLEDQGVYKAMNEFDIFINC IEAYMMIKMKS |
| Endotoxin : |
Less than 1 EU/mg of rMuIL-10 as determined by LAL method. |
| Purity : |
>97% by SDS-PAGE and HPLC analyses. |
| Storage : |
This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 to -70 centigrade. Avoid repeated freeze/thaw cycles. |
| Storage Buffer : |
Lyophilized from a 0.2mm filtered concentrated solution in PBS, pH 7.4. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at < -20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
