Species : |
Cotton Rat |
Source : |
E.coli |
Tag : |
Non |
Protein Length : |
1-160 a.a. |
Predicted N Terminal : |
Ser1 |
Form : |
Lyophilized from a 0.2 µm filtered solution in PBS with Trehalose and with BSA as a carrier protein. |
Bio-activity : |
Measured in a cell proliferation assay using MC/9-2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507.The ED50 for this effect is typically 0.04-0.24 ng/mL. |
Molecular Mass : |
Recombinant Cotton Rat Il10 has a calculated MW of 18.7 kDa (monomer). |
Purity : |
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
Storage : |
Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC). |
Reconstitution : |
Reconstitute at 10 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |