Active Recombinant Mouse Il11 Protein (179 aa)
|Product Overview :||Recombinant Mouse Il11 Protein without tag was expressed in E. coli.|
- Gene Information
- Related Products
|Description :||Interleukin 11 is a pleiotropic cytokine that was originally detected in the conditioned medium of an IL-1α-stimulated primate bone marrow stromal cell line (PU-34) as a mitogen for the IL-6-responsive murine plasmacytoma cell line T11. IL-11 contains no cysteine residues or potential glycosylation sites. IL-11 has multiple effects on both hematopoietic and nonhematopoietic cells. Many of the biological effects described for IL-11 overlap those for IL-6. In vitro, IL-11 can synergize with IL-3, IL-4 and SCF to shorten the G0 period of early hematopoietic progenitors. IL-11 also enhances the IL-3-dependent megakaryocyte colony formation. IL-11 has been found to stimulate the T cell dependent development of specific immunoglobulin-secreting B cell.|
|Source :||E. coli|
|Form :||Sterile Filtered White lyophilized (freeze-dried) powder.|
|Bio-activity :||Fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of the proliferation of murine T11 was found to be less than 2.0 ng/mL, corresponding to a specific activity of ＞5 × 10^5 IU/mg.|
|Molecular Mass :||Approximately 19.1 kDa, a single non-glycosylated polypeptide chain containing 179 amino acids.|
|Protein Length :||179|
|AA Sequence :||MPGPPAGSPRVSSDPRADLDSAVLL TRSLLADTRQLAAQMRDKFPADGDH SLDSLPTLAMSAGTLGSLQLPGVLT RLRVDLMSYLRHVQWLRRAGGPSLK TLEPELGALQARLERLLRRLQLLMS RLALPQAAPDQPVIPLGPPASAWGS IRAAHAILGGLHLTLDWAVRGLLLL KTRL|
|Endotoxin :||Less than 1 EU/mg of rmIL-11 as determined by LAL method.|
|Purity :||>97% by SDS-PAGE and HPLC analyses.|
|Storage :||This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 to -70 centigrade. Avoid repeated freeze/thaw cycles.|
|Storage Buffer :||Lyophilized from a 0.2mm filtered solution in PBS, pH 7.4.|
|Reconstitution :||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 centigrade. Further dilutions should be made in appropriate buffered solutions.|
|Gene Name :||Il11 interleukin 11 [ Mus musculus ]|
|Official Symbol :||Il11|
|Synonyms :||IL11; interleukin 11; interleukin-11; IL-11;|
|Gene ID :||16156|
|mRNA Refseq :||NM_008350|
|Protein Refseq :||NP_032376|
|UniProt ID :||P47873|
|◆ Recombinant Protein|
|Il11-138M||Recombinant Active Mouse IL11 Protein, His-tagged(N-ter)||+Inquiry|
|IL11-137H||Recombinant Active Human IL11 Protein, His-tagged(N-ter)||+Inquiry|
|IL11-131M||Active Recombinant Mouse IL11 Protein||+Inquiry|
|Il11-085M||Active Recombinant Mouse Il11 Protein||+Inquiry|
|IL11-061H||Active Recombinant Human IL11 Protein||+Inquiry|
|IL11-5249HCL||Recombinant Human IL11 293 Cell Lysate||+Inquiry|
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (10)Ask a question
Transgenic mice and knockdown models explore IL11's impact on tissue regeneration.
Site-directed mutagenesis identifies key residues in IL11's interaction with IL11RA.
Chromatin immunoprecipitation and DNA methylation analysis decipher IL11's regulatory mechanisms.
Proteomics identifies IL11-interacting proteins and potential cross-talk partners.
Cell-based and luciferase reporter assays elucidate IL11's JAK-STAT signaling activation.
RNA sequencing uncovers IL11-induced gene expression changes across different cell types.
X-ray crystallography and NMR spectroscopy reveal structural details of the IL11-IL11RA complex.
Confocal and super-resolution microscopy visualize IL11 localization in tissues.
Techniques like surface plasmon resonance measure IL11-IL11RA binding affinity and kinetics.
CRISPR screening unveils genes and pathways influenced by IL11 signaling.
Customer Reviews (3)Write a review
Potent in bone regeneration models. -
Suitable for cell culture. -
High yield in expression. -
Ask a Question for All Il11 Products
Required fields are marked with *
My Review for All Il11 Products
Required fields are marked with *