Active Recombinant Mouse IL18RAP Protein, His-tagged

Cat.No. : IL18RAP-922M
Product Overview : Recombinant mouse IL18RAP (NP_034683.1) (Met1-Glu356) was expressed with a C-terminal polyhistidine tag.
  • Specification
  • Gene Information
  • Related Products
Source : Baculovirus-Insect cells
Species : Mouse
Tag : His
Predicted N Terminal : Phe 20
Form : Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity : Measured by its binding ability in a functional ELISA. Immobilized mouse IL18RAP-His at 10 μg/ml (100 μl/well) can bind biotinylated mouse IL18, The EC50 of biotinylated mouse IL18 is 10.6-25.0 μg/ml.
Molecular Mass : The recombinant mouse IL18RAP comprises 348 amino acids and has a predicted molecular mass of 40.1 kDa. The apparent molecular mass of the protein is approximately 44 kDa in SDS-PAGE under reducing conditions.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >95 % as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : Il18rap interleukin 18 receptor accessory protein [ Mus musculus ]
Official Symbol : IL18RAP
Synonyms : IL18RAP; interleukin 18 receptor accessory protein; interleukin-18 receptor accessory protein; IL-18RAcP; IL-1RAcPL; IL-18Rbeta; IL-18R-beta; AcPL accessory protein-like); interleukin-18 receptor beta; IL-18 receptor accessory protein; CD218 antigen-like
Gene ID : 16174
mRNA Refseq : NM_010553
Protein Refseq : NP_034683
MIM :
UniProt ID :
Pathway : Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem;
Function : interleukin-1 receptor activity; receptor activity; transmembrane signaling receptor activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (10)

Ask a question
What experimental methods are commonly employed to study the expression patterns and regulation of IL18RAP across different cell types and conditions? 12/01/2021

RT-qPCR, immunohistochemistry, and single-cell RNA sequencing characterize IL18RAP expression levels and regulation in diverse cellular contexts.

What techniques are utilized to investigate the role of IL18RAP in immune responses, inflammation, and diseases, and how do researchers assess its potential as a therapeutic target? 03/15/2021

Animal models, patient samples, and therapeutic interventions elucidate IL18RAP's involvement in immune-related disorders and its potential as a therapeutic target.

Could you provide insights into the experimental approaches used to explore the functional effects of IL18RAP isoforms and their specific contributions to IL18 signaling? 12/21/2020

Isoform-specific knockdown, mutagenesis, and functional assays delineate IL18RAP isoforms' distinct roles in modulating IL18-mediated responses.

How are co-immunoprecipitation, fluorescence resonance energy transfer (FRET), and biophysical assays applied to characterize the interactions between IL18RAP, IL18, and IL18R1? 12/20/2019

Co-immunoprecipitation assays, FRET studies, and biophysical techniques characterize IL18RAP's interactions with IL18 and IL18R1 partners.

What experimental methods are used to elucidate the effects of post-translational modifications, such as glycosylation, on IL18RAP's structure and function? 05/18/2019

Mass spectrometry, mutagenesis, and functional assays investigate the impact of post-translational modifications on IL18RAP's conformation and function.

How do researchers experimentally determine the binding affinity and kinetics of IL18RAP to IL18 and IL18R1, considering its pivotal role in forming the IL18 receptor complex? 11/17/2018

Surface plasmon resonance, bio-layer interferometry, and surface plasmon-enhanced fluorescence assays quantify IL18RAP's binding kinetics to IL18 and IL18R1.

Can you explain the use of knockdown, knockout, and gene editing techniques to uncover the physiological importance of IL18RAP in mediating IL18 signaling? 08/10/2018

siRNA knockdown, CRISPR/Cas9-mediated knockout, and transgenic models unravel IL18RAP's physiological roles and contributions to IL18 signaling.

How are advanced imaging techniques, such as single-molecule imaging and super-resolution microscopy, utilized to visualize the dynamic assembly of the IL18 receptor complex involving IL18RAP? 11/05/2017

Single-molecule imaging, super-resolution microscopy, and live-cell imaging visualize the dynamic assembly of IL18 receptor complexes involving IL18RAP.

Can you elaborate on the strategies used to investigate the structural features of IL18RAP's interaction interfaces with IL18 and IL18R1, and how these interfaces contribute to receptor activation? 10/29/2017

X-ray crystallography, NMR spectroscopy, and computational modeling reveal IL18RAP's structural interactions with IL18 and IL18R1 domains.

How do researchers dissect the intracellular signaling pathways triggered by IL18RAP upon binding to IL18 and its collaboration with IL18R1? 10/09/2017

Phosphoprotein analysis, pathway-specific inhibitors, and functional assays dissect IL18RAP's intracellular signaling cascades and its crosstalk with IL18R1.

Customer Reviews (3)

Write a review
Reviews
02/09/2021

    Effective in signaling studies.

    11/17/2020

      High specificity.

      09/16/2018

        Reliable in co-immunoprecipitation.

        Ask a Question for All IL18RAP Products

        Required fields are marked with *

        My Review for All IL18RAP Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends