Active Recombinant Mouse Il1a Protein
|Product Overview :||Purified recombinant protein of Mouse interleukin 1 alpha (Il1a) (Ser115-Ser270) without tag was expressed in E.coli.|
- Gene Information
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|Description :||Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.|
|Source :||E. coli|
|Bio-activity :||The ED50 is 1- 5 pg/ml, corresponding to a specific activity of 1- 0.2 x 10^9 units/mg, determined by the dose dependent stimulation of D10S cells proliferation|
|Molecular Mass :||18 kDa|
|Endotoxin :||Less than 0.01 ng per µg protein as determined by the LAL method|
|Purity :||>98%, as determined by Coomassie stained SDS-PAGE.|
|Stability :||Unopened vial can be stored between 2 centigrade and 8 centigrade for up to 2 weeks, at -20 centigrade for up to 6 months, or at -70 centigrade or below until the expiration date. Aliquots can be stored between 2 centigrade and 8 centigrade for up to one week and stored at -20 centigrade or colder for up to 3 months. Avoid repeated freeze/thaw cycles.|
|Storage :||Store at -80 centigrade.|
|Concentration :||Protein formulated and vialed at >50 ug/mL in specified buffer|
|Storage Buffer :||10mM NaH2PO4, 150mM NaCl, pH7.2|
|Gene Name :||Il1a interleukin 1 alpha [ Mus musculus (house mouse) ]|
|Official Symbol :||Il1a|
|Synonyms :||Il1a; interleukin 1 alpha; Il-1a; interleukin-1 alpha; IL-1 alpha|
|Gene ID :||16175|
|mRNA Refseq :||NM_010554|
|Protein Refseq :||NP_034684|
|UniProt ID :||P01582|
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Q&As (10)Ask a question
Cross-talk between IL-1α's and other cytokines is dissected through methods such as co-immunoprecipitation, ELISA, and cytokine profiling arrays.
IL-1α's paradoxical roles are dissected using conditional knockout mice, 3D culture systems, and molecular analyses to delineate context-specific effects.
Single-cell RNA sequencing uncovers heterogeneity in IL-1α-responsive cell populations and their roles, revealing distinct molecular profiles and functions.
IL-1α's intricate pro-inflammatory signaling pathways are decoded using advanced techniques like mass spectrometry, phosphoproteomics, and bioinformatics.
Genetic editing tools like CRISPR-Cas9 unveil IL-1α's contributions to diseases by generating knockout models and studying resulting phenotypic changes.
In vitro co-culture systems are manipulated to study IL-1α-mediated responses, elucidating immune-stromal cell interactions via cytokine-specific neutralization.
Specific cellular receptors and effectors influenced by IL-1α are identified through techniques like co-immunoprecipitation, ChIP-seq, and siRNA knockdown.
Live-cell microscopy captures real-time IL-1α release dynamics and its impact on neighboring cells, facilitated by fluorescent tagging and high-resolution imaging.
Longitudinal studies and statistical modeling establish the quantitative relationship between IL-1α levels and disease progression, offering predictive insights.
Multi-omics analyses provide a holistic view of IL-1α signaling's impact, integrating transcriptomics, proteomics, and metabolomics data for comprehensive insights.
Customer Reviews (3)Write a review
High solubility. -
Good for intracellular staining. -
Effective in apoptosis assays. -
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