Active Recombinant Mouse Il2 Protein (149 aa)

• Cat.No.: Il2-028I
• Product Overview: Recombinant Mouse Il2 Protein without tag was expressed in E. coli.

Specification

• Description: Mature mouse IL-2 shares 56% and 73% aa sequence identity with human and rat IL-2, respectively. It shows strain-specific heterogeneity in an N-terminal region that contains a poly-glutamine stretch. Mouse and human IL-2 exhibit cross-species activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. The 55 kDa IL-2 Rαis specific for IL-2 and binds with low affinity. The 75 kDa IL-2 Rβ, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chainγc/IL-2 Rγ, which is shared with the receptors for IL-4, -7, -9, -15, and -21, does not independently interact with IL-2. Upon ligand binding, signal transduction is performed by both IL-2 Rβandγc. It drives resting T cells to proliferate and induces IL-2 and IL-2 Rαsynthesis. It contributes to T cell homeostasis by promoting the Fas-induced death of naive CD4+ T cells but not activated CD4+ memory lymphocytes. IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells.
• Source: E. coli
• Species: Mouse
• Form: Sterile Filtered White lyophilized (freeze-dried) powder.
• Bio-activity: The ED50 as determined by the dose dependent stimulation of murine CTLL-2 cells is < 0.2 ng/mL, corresponding to a specific activity of > 5 × 10^6 units/mg.
• Molecular Mass: Approximately 17.2 kDa, a single non-glycosylated polypeptide chain containing 149 amino acids.
• Protein Length: 149
• AA Sequence: PTSSSTSSSTAEAQQQQQQQQQQQQ HLEQLLMDLQELLSRMENYRNLKLP RMLTFKFYLPKQATELKDLQCLEDE LGPLRHVLDLTQSKSFQLEDAENFI SNIRVTVVKLKGSDNTFECQFDDES ATVVDFLRRWIAFCQSIISTSPQ
• Endotoxin: Less than 1 EU/mg of rmIL-2 as determined by LAL method.
• Purity: >95% by SDS-PAGE and HPLC analyses.
• Storage: This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 to -70 centigrade. Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2mm filtered solution in PBS, pH 7.4.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 centigrade. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: Il2 interleukin 2 [ Mus musculus ]
• Official Symbol: Il2
• Synonyms: IL2; interleukin 2; interleukin-2; TCGF; T-cell growth factor; Il-2
• Gene ID: 16183
• mRNA Refseq: NM_008366
• Protein Refseq: NP_032392
• UniProt ID: P04351