Il1r1-5652M
Optional Service: Optional requirements on this protein
Product Overview :
RecombinantMouse Il1r1 protein was expressed in Murine myeloma cellline.Asp22-Arg338 (Ala158Val), with a C-terminal 6-His tag.
Description :
Il1r1 isa member of the IL-1 R/TLR superfamily. Members of this superfamily have a characteristicToll-IL-1 R (TIR) domain in their cytoplasmic region. This superfamily can befurther divided into three subgroups based on extracellular region analogies.Il1r1 belongs to Subgroup 1 which is characterized by the presence of atleast one immunoglobulin-like domain. Mouse Il1r1 is a 70 kDa type I transmembraneglycoprotein that is synthesized as a 574 amino acid (aa) precursor. Theprecursor contains a 21 aa signal sequence, a 317 aa extracellular region, a21 aa transmembrane segment, and a 215 aa cytoplasmic domain.
Source :
Mammalian cells
Form :
Lyophilizedfrom a 0.2 um filtered solution in PBS with BSA as a carrier protein.
N-terminalSequence :
Asp22
MolecularWeight :
36.8 kDa
Activity :
Measuredby its binding ability in a functional ELISA. Immobilized rmIL-1F5, rmIL-1F6,or rmIL-1F8 at 1 μg/mL (100 μL/well) can bind rm Il1r1/his witha linear range of 0.078-5 μg/mL.
Endotoxin Level :
<1.0EU per 1μg of the protein by the LAL method.
SDS-PAGE :
55-68kDa, reducing conditions.
Purity :
>90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution :
100 μg/mLin sterile PBS containing at least 0.1% human or bovine serum albumin
Storage :
Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.