Recombinant Human IL1R1 protein, His-tagged

• Cat.No.: IL1R1-552H

Specification

• Product overview: Recombinant Human IL1R1 (P14778) aa. (Asp21~Ile109) fused with His tag was produced in E. coli cells.
• Description: This gene encodes a cytokine receptor that belongs to the interleukin-1 receptor family. The encoded protein is a receptor for interleukin-1 alpha, interleukin-1 beta, and interleukin-1 receptor antagonist. It is an important mediator involved in many cytokine-induced immune and inflammatory responses. This gene is located in a cluster of related cytokine receptor genes on chromosome 2q12.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 11.6kDa; Accurate Molecular Mass: 14kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Asp21~Ile109
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: Isoelectric point is 7.8.
• Applications: Cell culture; Activity Assays; In vivo assays.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: 20mM Tris, 150mM NaCl,pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

Gene Information

• Gene Name: IL1R1 interleukin 1 receptor type 1 [ Homo sapiens (human) ]
• Official Symbol: IL1R1
• Synonyms: IL1R1; P80; IL1R; IL1RA; CD121A; D2S1473; IL-1R-alpha; interleukin-1 receptor type 1; CD121 antigen-like family member A; IL-1R-1; IL-1RT-1; IL-1RT1; interleukin 1 receptor alpha, type I; interleukin-1 receptor alpha; interleukin-1 receptor type I
• Gene ID: 3554
• mRNA Refseq: NM_000877.3
• Protein Refseq: NP_000868.1
• UniProt ID: P14778

Reviews

07/14/2022

High stability.

06/07/2017

Suitable for ELISA.

04/19/2017

Effective in signaling studies.

Q&As

How does the cellular localization of IL1R1 influence its responsiveness to ligands, and how can live-cell imaging techniques track its dynamic movement on the cell surface?

Live-cell imaging techniques track IL1R1's movement on the cell surface, revealing how cellular localization impacts its responsiveness to ligands.

How can advanced computational modeling and molecular dynamics simulations predict the allosteric changes in IL1R1's intracellular domain upon ligand binding, offering insights into its downstream signaling cascade?

Advanced computational modeling and molecular dynamics simulations predict allosteric changes in IL1R1's intracellular domain upon ligand binding, revealing insights into its downstream signaling cascade.

What are the mechanisms underlying IL1R1's dimerization and oligomerization, and how can FRET and cross-linking assays provide insights into its multimeric assembly?

FRET and cross-linking assays reveal the multimeric assembly of IL1R1, shedding light on its dimerization and oligomerization dynamics.

How does IL1R1's endocytosis and recycling affect its signaling duration, and how can confocal microscopy and flow cytometry unveil its intracellular trafficking patterns?

Confocal microscopy and flow cytometry visualize IL1R1's endocytosis and recycling, offering insights into its intracellular trafficking dynamics and signaling duration.

What are the intricate molecular details of IL1R1's recruitment of MyD88 and other adapter proteins upon ligand binding, and how can co-immunoprecipitation assays reveal these interactions?

Co-immunoprecipitation assays unravel the interactions between IL1R1 and adapter proteins, elucidating the molecular details of its intracellular signaling cascade.

How does the interaction between IL1R1 and accessory proteins like IL1RAP influence its ligand binding and intracellular signaling, and how can mutagenesis studies dissect these interfaces?

Mutagenesis studies dissect the interfaces between IL1R1 and accessory proteins like IL1RAP, uncovering their roles in ligand binding and signaling modulation.

How do the structural dynamics of IL1R1's extracellular domains modulate its binding to various ligands, and how can cryo-electron microscopy unveil these conformational changes?

Cryo-electron microscopy captures IL1R1's structural shifts during ligand binding, unveiling conformational changes that influence its ligand recognition.

What are the intricate crosstalk mechanisms between IL1R1 and other receptors in cell signaling networks, and how can proteomics-based studies dissect these interconnected pathways?

Proteomics-based studies uncover the crosstalk between IL1R1 and other receptors in signaling networks, unveiling their interconnected pathways.

What are the complex regulatory mechanisms governing IL1R1's expression and alternative splicing, and how can CRISPR-based approaches shed light on the functional consequences of these variations?

CRISPR-based approaches manipulate IL1R1 expression and alternative splicing, providing functional insights into the complex regulatory mechanisms governing its variations.

In what ways does IL1R1's post-translational modification landscape impact its downstream signaling, and how can phosphoproteomics offer a comprehensive view of its phosphorylation events?

Phosphoproteomics characterizes IL1R1's phosphorylation landscape, revealing how post-translational modifications shape its downstream signaling outcomes.