| Cat. No. : |
Il1b-463R |
| Description : |
Interleukin-1beta (IL-1μ) is a proinflammatory cytokine produced in response to inflammatory agents by a variety of cells, including monocytes, macrophages, and dendritic cells (DCs). IL-1μ and IL-1μ are two distinct and independently regulated gene products that comprise IL-1 and signal through the Type 1 IL-1 receptor (IL-1R1). Although IL-1μ is cell associated and IL-1μ is secreted, they have nearly identical biological activity in that they induce adhesion molecule expression on epithelial cells, control fever induction, and play a role in arthritis and septic shock. Signaling activated by the IL-1R1 promotes these activities through a MYD88 signaling pathway similar to those associated with Toll receptors. |
| Species : |
Rat |
| Source : |
E.coli |
| Form : |
Lyopohilized from 10 mM NaP, pH 7.5. |
| Purity : |
Purity Greater than 97% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. |
| Bio-activity : |
Rat IL-1b is fully biologically active when compared to a standard. The ED50, as determined by the dose-dependent proliferation of mouse D10S cells, is 40.2 pg/ml. |
| Physical Appearance : |
Sterile Filtered white lyophilized (freeze-dried) powder. |
| Solubility : |
It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mgμs/ml, which can be further diluted into other aqueous solutions. |
| Purity Greater than 97% as determined by : |
Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE. |
| Protein Content determined by : |
UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard. |
| Endotoxin Level : |
Endotoxin level is <0.01ng/ug or <0.1EU/ug. |
| Amino acid sequence : |
VPIRQLHCRLRDEQQKCLVLSDPCE LKALHLNGQNISQQVVFSMSFVQGE TSNDKIPVALGLKGLNLYLSCVMKD GTPTL QLESVDPKQYPKKKMEKRFVFNKIE VKTKVEFESAQFPNWYISTSQAEHR PVFLGNSNGRDIVDFTMEPVSS |
| Storage : |
Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage. |
