DEAE Cellulose Resin
Cat.No. : | diethylaminoethyl-004C |
Product Overview : | DEAE-Cellulose is a cellulose ion-exchange adsorbent used in ion exchange chromatography. |
- Specification
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Description : | CAS number is 9013-34-7. MDL number: MFCD00146622 |
Form : | Solid |
Usage : | Provided as a dry resin. The following procedure should be carried out in a Buchner funnel: Suspend dry resin in 5 volumes diH2O and allow to swell and settle for 30-45 minutes. Measure volume of settled resin to determine Column Volume (CV) for washing steps. For activation/regeneration: 1, Filter suspension and re-suspend resin in 2 CV of 0.1M NaOH/0.5M NaCl for 10 minutes. Pour slurry into Buchner funnel and allow buffer to slowly drain off (~1 V buffer in 5 minutes); 2, Repeat using 0.5M NaCl (without 0.1M NaOH); 3, Repeat using 0.1M HCl/0.5 M NaCl; 4, Repeat using diH2O; 5, Continue washing with diH2O until effluent pH is 5 or greater (~5-10 CV); 6, Suspend the resin in 2 CV of 1M NaCl and adjust pH to 7-8 with NaOH and store at 4°C. To Use Resin: 1, Filter resin and pass 5 CV of diH2O through resin; 2, Resuspend in 2 CV of 10X buffer of your choice and filter; 3, Remove resin from funnel and re-suspend with 5 CV of 1X buffer and filter. Wash with 1X buffer until pH is within 0.15 pH units of the 1X buffer; 4, Pack resin into column via gravity flow; 5, Apply sample, wash resin, and elute sample. |
Storage : | Store at 4 centigrade. |
Synonyms : | Diethylaminoethyl-cellulose |
Synonyms : | Diethylaminoethyl-cellulose |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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