Expand| Cat. No. : | Gdf5-720M |
| Product Overview : | Recombinant Mouse Gdf5protein was expressed in E. coli with and without an N-terminal Met. Ala376-Arg495. |
| Description : | Gdf5, also known ascartilage-derived morphogenetic protein 1, is a member of the bonemorphogenetic protein (BMP) family which belongs to the transforming growthfactor β (TGFβ) superfamily. Gdf5 is synthesized as a large precursor proteinthat consists of an N-terminal 19 amino acid (aa) signal sequence, a 362 aapro region and a 120 aa C-terminal mature peptide. Mature Gdf5 isahomodimeric protein which contains the characteristic seven conservedcysteine residues. |
| Source : | E. coli-derived. |
| Form : | Lyophilized from a0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrierprotein. |
| N-terminalSequence : | Met and Ala376 |
| Molecular Weight : | 13.7 kDa |
| Activity : | Measuredby its ability to induce alkaline phosphatase production by ATDC5 mousechondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50for this effect is typically 0.2-1.2 μg/mL. |
| Endotoxin Level : | <1.0 EUper 1 μg of the protein by the LAL method. |
| Purity : | >95%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Reconstitution : | 150μg/mL in sterile 4 mM HCl that containing at least 0.1% human or bovine serumalbumin. |
| Storage : | Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution. |
| Gene Name : | Gdf5 growth differentiationfactor 5 [ Mus musculus ] |
| Official Symbol : | Gdf5 |
| Synonyms : | Gdf5; growthdifferentiation factor 5; bp; brp; Cdmp-1; growth/differentiation factor 5;GDF-5; brachypodism; cartilage-derived morphogenetic protein-1 |
| Gene ID : | 14563 |
| mRNA Refseq : | NM_008109 |
| Protein Refseq : | NP_032135 |
| UniProt ID : | P43027 |
| Chromosome Location : | 2 H1; 2 90.0 cM |
| Pathway : | Cytokine-cytokinereceptor interaction; TGF-beta signaling pathway |
| Function : | cytokine activity;growth factor activity; protein binding |
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