Recombinant Mouse Growth Differentiation Factor 5 Gdf5-720M

Specification
Gene Information
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Cat. No.:	Gdf5-720M
Product Overview:	Recombinant Mouse Gdf5protein was expressed in E. coli with and without an N-terminal Met. Ala376-Arg495.
Description:	Gdf5, also known ascartilage-derived morphogenetic protein 1, is a member of the bonemorphogenetic protein (BMP) family which belongs to the transforming growthfactor β (TGFβ) superfamily. Gdf5 is synthesized as a large precursor proteinthat consists of an N-terminal 19 amino acid (aa) signal sequence, a 362 aapro region and a 120 aa C-terminal mature peptide. Mature Gdf5 isahomodimeric protein which contains the characteristic seven conservedcysteine residues.
Source:	E. coli-derived.
Form:	Lyophilized from a0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrierprotein.
N-terminalSequence:	Met and Ala376
Molecular Weight:	13.7 kDa
Activity:	Measuredby its ability to induce alkaline phosphatase production by ATDC5 mousechondrogenic cells. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351. The ED50for this effect is typically 0.2-1.2 μg/mL.
Endotoxin Level:	<1.0 EUper 1 μg of the protein by the LAL method.
Purity:	>95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution:	150μg/mL in sterile 4 mM HCl that containing at least 0.1% human or bovine serumalbumin.
Storage:	Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.

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Gene Name:	Gdf5 growth differentiationfactor 5 [ Mus musculus ]
Official Symbol:	Gdf5
Synonyms:	Gdf5; growthdifferentiation factor 5; bp; brp; Cdmp-1; growth/differentiation factor 5;GDF-5; brachypodism; cartilage-derived morphogenetic protein-1
Gene ID:	14563
mRNA Refseq:	NM_008109
Protein Refseq:	NP_032135
UniProt ID:	P43027
Chromosome Location:	2 H1; 2 90.0 cM
Pathway:	Cytokine-cytokinereceptor interaction; TGF-beta signaling pathway
Function:	cytokine activity;growth factor activity; protein binding