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Active Recombinant Rat Beta-1,3-Glucuronyltransferase 1 (glucuronosyltransferase P), His-tagged

Cat.No. : B3gat1-590R
Product Overview : Recombinant Rat B3gat1protein was expressed in Murine myeloma cell line with an N-terminal humanCD33 signal sequence and a 6-His tag. Asp75-Ile347.
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  • Gene Information
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Cat. No. : B3gat1-590R
Description : B3gat1 is a keyenzyme involved in human natural killer-1 (HNK-1) epitope synthesis. It addsa glucuronic residue to the terminal lactosamine residue (Galβ1-4GlcNAc-) ofa glycoprotein or glycolipid, which can be further sulfated to become the HNK1 epitope, a unique trisaccharide structure, HSO3-3GlcAβ1-3Galβ1-4GlcNAc-.The enzyme activity was found to be enhanced in the presence of sphingomyelinand phosphatidylinositol.The B3gat1 carbohydrate epitope ischaracteristically expressed on a series of cell adhesion molecules in additionto some glycolipids in the extracellular matrix and on the cell surface inthe nervous system, where it is involved in cell-cell and cellsubstratum interactionand recognition during the development of the nervous system.
Source : Mammalian cells
Form : Supplied as a 0.2 μmfiltered solution in Tris, NaCl and DTT.
N-terminalSequence : His
Molecular Weight : 32 kDa
Activity : Measured by itsability to transfer GlcA from UDPGlcA to lactose. The specific activity is> 725 pmol/min/μg, as measured under the described conditions.
Endotoxin Level : < 1.0EU per 1 μg of the protein by the LAL method.
SDS-PAGE : 40-43kDa, reducing conditions.
Purity : > 80%, by SDSPAGEunder reducing conditions and visualized by Colloidal Coomassie® Blue stainat 5 μg per lane.
Reconstitution : 100 μg/mLin sterile PBS.
Storage : Use a manual defrostfreezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt,-20 to -70°C as supplied. 3 months, -20 to -70°C under sterile conditionsafter opening.
Gene Name : B3gat1beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) [ Rattusnorvegicus ]
Official Symbol : B3gat1
Synonyms : B3gat1;beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P); Hnk-1; galactosylgalactosylxylosylprotein3-beta-glucuronosyltransferase 1; glcAT-P; glcUAT-P; glucuronosyltransferaseP; glucuronosyltransferase-P; UDP-GlcUA:glycoproteinbeta-1,3-glucuronyltransferase; EC 2.4.1.135
Gene ID : 117108
mRNA Refseq : NM_054003
Protein Refseq : NP_446455
Chromosome Location : 8q13
Pathway : Glycosaminoglycanbiosynthesis - chondroitin sulfate; Glycosaminoglycan biosynthesis - heparansulfate; Glycosaminoglycan biosynthesis, linkage tetrasaccharide;Glycosaminoglycan biosynthesis; Metabolic pathways
Function : UDP-galactose:beta-N-acetylglucosaminebeta-1,3-galactosyltransferase activity; galactosylgalactosylxylosylprotein3-beta-glucuronosyltransferase activity; metal ion binding; transferaseactivity

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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