"B3gat1" Related Products


Recombinant Rat Beta-1,3-Glucuronyltransferase 1 (glucuronosyltransferase P), His-tagged

Cat. No.: B3gat1-590R
Product Overview: Recombinant Rat B3gat1protein was expressed in Murine myeloma cell line with an N-terminal humanCD33 signal sequence and a 6-His tag. Asp75-Ile347.
Description: B3gat1 is a keyenzyme involved in human natural killer-1 (HNK-1) epitope synthesis. It addsa glucuronic residue to the terminal lactosamine residue (Galβ1-4GlcNAc-) ofa glycoprotein or glycolipid, which can be further sulfated to become the HNK1 epitope, a unique trisaccharide structure, HSO3-3GlcAβ1-3Galβ1-4GlcNAc-.The enzyme activity was found to be enhanced in the presence of sphingomyelinand phosphatidylinositol.The B3gat1 carbohydrate epitope ischaracteristically expressed on a series of cell adhesion molecules in additionto some glycolipids in the extracellular matrix and on the cell surface inthe nervous system, where it is involved in cell-cell and cellsubstratum interactionand recognition during the development of the nervous system.
Source: Murine myeloma cellline, NS0-derived.
Form: Supplied as a 0.2 μmfiltered solution in Tris, NaCl and DTT.
N-terminalSequence: His
Molecular Weight: 32 kDa
Activity: Measured by itsability to transfer GlcA from UDPGlcA to lactose. The specific activity is> 725 pmol/min/μg, as measured under the described conditions.
Endotoxin Level: < 1.0EU per 1 μg of the protein by the LAL method.
SDS-PAGE: 40-43kDa, reducing conditions.
Purity: > 80%, by SDSPAGEunder reducing conditions and visualized by Colloidal Coomassie® Blue stainat 5 μg per lane.
Reconstitution: 100 μg/mLin sterile PBS.
Storage: Use a manual defrostfreezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt,-20 to -70°C as supplied. 3 months, -20 to -70°C under sterile conditionsafter opening.
Gene Name: B3gat1beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) [ Rattusnorvegicus ]
Official Symbol: B3gat1
Synonyms: B3gat1;beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P); Hnk-1; galactosylgalactosylxylosylprotein3-beta-glucuronosyltransferase 1; glcAT-P; glcUAT-P; glucuronosyltransferaseP; glucuronosyltransferase-P; UDP-GlcUA:glycoproteinbeta-1,3-glucuronyltransferase; EC
Gene ID: 117108
mRNA Refseq: NM_054003
Protein Refseq: NP_446455
Chromosome Location: 8q13
Pathway: Glycosaminoglycanbiosynthesis - chondroitin sulfate; Glycosaminoglycan biosynthesis - heparansulfate; Glycosaminoglycan biosynthesis, linkage tetrasaccharide;Glycosaminoglycan biosynthesis; Metabolic pathways
Function: UDP-galactose:beta-N-acetylglucosaminebeta-1,3-galactosyltransferase activity; galactosylgalactosylxylosylprotein3-beta-glucuronosyltransferase activity; metal ion binding; transferaseactivity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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