Active Recombinant Rat Beta-1,3-Glucuronyltransferase 1 (glucuronosyltransferase P), His-tagged

• Cat.No.: B3gat1-590R
• Product Overview: Recombinant Rat B3gat1protein was expressed in Murine myeloma cell line with an N-terminal humanCD33 signal sequence and a 6-His tag. Asp75-Ile347.

Specification

• Species: Rat
• Source: Mammalian Cells
• Tag: His
• Protein Length: 75-347 a.a.
• Description: B3gat1 is a keyenzyme involved in human natural killer-1 (HNK-1) epitope synthesis. It addsa glucuronic residue to the terminal lactosamine residue (Galβ1-4GlcNAc-) ofa glycoprotein or glycolipid, which can be further sulfated to become the HNK1 epitope, a unique trisaccharide structure, HSO3-3GlcAβ1-3Galβ1-4GlcNAc-.The enzyme activity was found to be enhanced in the presence of sphingomyelinand phosphatidylinositol.The B3gat1 carbohydrate epitope ischaracteristically expressed on a series of cell adhesion molecules in additionto some glycolipids in the extracellular matrix and on the cell surface inthe nervous system, where it is involved in cell-cell and cellsubstratum interactionand recognition during the development of the nervous system.
• Form: Supplied as a 0.2 μmfiltered solution in Tris, NaCl and DTT.
• N-terminalSequence: His
• Molecular Weight: 32 kDa
• Activity: Measured by itsability to transfer GlcA from UDPGlcA to lactose. The specific activity is> 725 pmol/min/μg, as measured under the described conditions.
• Endotoxin Level: < 1.0EU per 1 μg of the protein by the LAL method.
• SDS-PAGE: 40-43kDa, reducing conditions.
• Purity: > 80%, by SDSPAGEunder reducing conditions and visualized by Colloidal Coomassie® Blue stainat 5 μg per lane.
• Reconstitution: 100 μg/mLin sterile PBS.
• Storage: Use a manual defrostfreezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt,-20 to -70°C as supplied. 3 months, -20 to -70°C under sterile conditionsafter opening.

Gene Information

• Gene Name: B3gat1beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) [ Rattusnorvegicus ]
• Official Symbol: B3gat1
• Synonyms: B3gat1;beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P); Hnk-1; galactosylgalactosylxylosylprotein3-beta-glucuronosyltransferase 1; glcAT-P; glcUAT-P; glucuronosyltransferaseP; glucuronosyltransferase-P; UDP-GlcUA:glycoproteinbeta-1,3-glucuronyltransferase; EC 2.4.1.135
• Gene ID: 117108
• mRNA Refseq: NM_054003
• Protein Refseq: NP_446455
• Chromosome Location: 8q13
• Pathway: Glycosaminoglycanbiosynthesis - chondroitin sulfate; Glycosaminoglycan biosynthesis - heparansulfate; Glycosaminoglycan biosynthesis, linkage tetrasaccharide;Glycosaminoglycan biosynthesis; Metabolic pathways
• Function: UDP-galactose:beta-N-acetylglucosaminebeta-1,3-galactosyltransferase activity; galactosylgalactosylxylosylprotein3-beta-glucuronosyltransferase activity; metal ion binding; transferaseactivity