||Recombinant mouseepidermal growth factor (EGF) is a 8.6 kDa globular protein containing 77amino acids residues, including 3 intra-molecular disulfide bonds. Recombinantmouse EGF protein, fused to His-tag at N-terminus, was expressed in E.coliand purified by conventional column chromatography, after refolding of theisolated inclusion bodies in a renaturation buffer.
||EGF is a potentgrowth factor that stimulates the proliferation of various epidermal andepithelial cells. Additionally, EGF has been shown to inhibit gastricsecretion, and to be involved in wound healing. EGF signals through areceptor known as c-erbB, which is a class I tyrosine kinase receptor.
||Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 100mM NaCl
||8.6kDa (77aa),confirmed by MALDI-TOF
||> 90% by SDS -PAGE
||0.5mg/ml (determinedby Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSMNSYPGC PSSYDGYCLN GGVCMHIESL DSYTCNCVIG YSGDRCQTRD LRWWELR
||Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles.