||Recombinant humanUBE2H protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography techniques.
||UBE2H(Ubiquitin-conjugating enzyme E2 H) belongs to the ubiquitin-conjugatingenzyme family. The modification of proteins with ubiquitin is an importantcellular mechanism for targeting abnormal or short-lived proteins fordegradation. Ubiquitination involves at least three classes of enzymes:ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s,and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2ubiquitin-conjugating enzyme family. UBE2H is Accepts ubiquitin from the E1complex and catalyzes its covalent attachment to other proteins.
||Liquid. In 20 mMTris-HCl buffer, pH8.0, 10% glycerol, 1mM DTT, 50mM NaCl.
||23.1 kDa(206aa) confirmed by MALDI-TOF.
||> 95%by SDS-PAGE
||1 mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSMSSPSPG KRRMDTDVVK LIESKHEVTI LGGLNEFVVK FYGPQGTPYEGGVWKVRVDL PDKYPFKSPS IGFMNKIFHP NIDEASGTVC LDVINQTWTA LYDLTNIFES FLPQLLAYPNPIDPLNGDAA AMYLHRPEEY KQKIKEYIQK YATEEALKEQ EEGTGDSSSE SSMSDFSEDE AQDMEL
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.