Recombinant Human Ubiquitin-Conjugating Enzyme E2H, His-tagged

• Cat.No.: UBE2H-1959H
• Product Overview: Recombinant humanUBE2H protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: UBE2H(Ubiquitin-conjugating enzyme E2 H) belongs to the ubiquitin-conjugatingenzyme family. The modification of proteins with ubiquitin is an importantcellular mechanism for targeting abnormal or short-lived proteins fordegradation. Ubiquitination involves at least three classes of enzymes:ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s,and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2ubiquitin-conjugating enzyme family. UBE2H is Accepts ubiquitin from the E1complex and catalyzes its covalent attachment to other proteins.
• Form: Liquid. In 20 mMTris-HCl buffer, pH8.0, 10% glycerol, 1mM DTT, 50mM NaCl.
• Molecular Weight: 23.1 kDa(206aa) confirmed by MALDI-TOF.
• Purity: > 95%by SDS-PAGE
• Concentration: 1 mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSMSSPSPG KRRMDTDVVK LIESKHEVTI LGGLNEFVVK FYGPQGTPYEGGVWKVRVDL PDKYPFKSPS IGFMNKIFHP NIDEASGTVC LDVINQTWTA LYDLTNIFES FLPQLLAYPNPIDPLNGDAA AMYLHRPEEY KQKIKEYIQK YATEEALKEQ EEGTGDSSSE SSMSDFSEDE AQDMEL
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: UBE2H ubiquitin-conjugatingenzyme E2H [ Homo sapiens ]
• Official Symbol: UBE2H
• Synonyms: UBE2H; ubiquitin-conjugating enzyme E2H;UBC8; UbcH2; UBCH; UBCH2; E2-20K; ubiquitin-conjugating enzyme E2 H;ubiquitin-protein ligase H; ubiquitin carrier protein H;ubiquitin-conjugating enzyme E2-20K; ubiquitin-conjugating enzyme E2H (UBC8homolog, yeast); ubiquitin-conjugating enzyme E2H (homologous to yeast UBC8);EC 6.3.2.19; OTTHUMP00000212335; OTTHUMP00000212338; OTTHUMP00000212339;OTTHUMP00000212355
• Gene ID: 7328
• mRNA Refseq: NM_003344
• Protein Refseq: NP_003335
• MIM: 601082
• UniProt ID: P62256
• Chromosome Location: 7q32
• Pathway: Adaptive Immune System; Antigen processing;Class I MHC mediated antigen processing & presentation; Immune System;Ubiquitin mediated proteolysis
• Function: ATP binding; acid-amino acid ligaseactivity; ligase activity; nucleotide binding; ubiquitin-protein ligaseactivity