Recombinant Human Integrin-binding Sialoprotein, His-tagged
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| Cat. No. : | IBSP-2711H |
| Product Overview : | Recombinant Human Bone Sialoprotein (Phe17Gln317) fused a His-tag at C-terminal was expressed in CHO cells. |
| Description : | Bone sialoprotein (BSP) is a component of mineralized tissues such as bone, dentin, cementum and calcified cartilage. BSP is a significant component of the bone extracellular matrix and has been suggested to constitute approximately 8% of all non-collagenous proteins found in bone and cementum. |
| Source : | CHO Cells |
| Species : | Human |
| Predicted NTerminal : | Phe17 |
| SDS-PAGE : | 7590 kDa, reducing conditions |
| Activity : | Measured by the ability of the immobilized protein to support the adhesion of the MCF-7human breast cancer cells. When 5 x104 cells/well are added to recombinant human IBSP- coated plates (3 µg/mL with 100 µL/well), approximately 60-80% will l adhereafter 30 minutes at 37°C. |
| Endotoxin : | Less than 1.0 EU per 1 μg of the protein by the LAL method. |
| Purity : | >90%, by SDSPAGE under reducing conditions and visualized by silver stain. |
| Formulation : | Lyophilized from a 0.2 μm filtered solution in MES and NaCl. See Certificate of Analysis for details. |
| Reconstitution : | Reconstitute at 100 μg/mL in sterile PBS. |
| Storage : | 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution. Avoid repeated freeze¬thaw cycles. |
| Gene Name : | IBSP integrin-binding sialoprotein [ Homo sapiens ] |
| Official Symbol : | IBSP |
| Synonyms : | integrin-binding sialoprotein; BSP; BNSP; SP-II; BSP-II; IBSP; bone sialoprotein 2; BSP II; bone sialoprotein II; cell-binding sialoprotein; Cell-binding sialoprotein; Bone sialoprotein II |
| Gene ID : | 3381 |
| mRNA Refseq : | NM_004967 |
| Protein Refseq : | NP_004958 |
| MIM : | 147563 |
| UniProt ID : | P21815 |
| Chromosome Location : | 4q21-q25 |
| Pathway : | ECM-receptor interaction; Focal adhesion; Integrin cell surface interactions |
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