Active Recombinant Human Integrin-binding Sialoprotein, His-tagged

• Cat.No.: IBSP-2711H
• Product Overview: Recombinant Human Bone Sialoprotein (Phe17­Gln317) fused a His-tag at C-terminal was expressed in CHO cells.

Specification

• Species: Human
• Source: CHO
• Tag: His
• Description: Bone sialoprotein (BSP) is a component of mineralized tissues such as bone, dentin, cementum and calcified cartilage. BSP is a significant component of the bone extracellular matrix and has been suggested to constitute approximately 8% of all non-collagenous proteins found in bone and cementum.
• Predicted NTerminal: Phe17
• SDS-PAGE: 75­90 kDa, reducing conditions
• Activity: Measured by the ability of the immobilized protein to support the adhesion of the MCF-7human breast cancer cells. When 5 x104 cells/well are added to recombinant human IBSP- coated plates (3 µg/mL with 100 µL/well), approximately 60-80% will l adhereafter 30 minutes at 37°C.
• Endotoxin: Less than 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS­PAGE under reducing conditions and visualized by silver stain.
• Formulation: Lyophilized from a 0.2 μm filtered solution in MES and NaCl. See Certificate of Analysis for details.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Storage: 12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution. Avoid repeated freeze¬thaw cycles.

Gene Information

• Gene Name: IBSP integrin-binding sialoprotein [ Homo sapiens ]
• Official Symbol: IBSP
• Synonyms: integrin-binding sialoprotein; BSP; BNSP; SP-II; BSP-II; IBSP; bone sialoprotein 2; BSP II; bone sialoprotein II; cell-binding sialoprotein; Cell-binding sialoprotein; Bone sialoprotein II
• Gene ID: 3381
• mRNA Refseq: NM_004967
• Protein Refseq: NP_004958
• MIM: 147563
• UniProt ID: P21815
• Chromosome Location: 4q21-q25
• Pathway: ECM-receptor interaction; Focal adhesion; Integrin cell surface interactions