Cat. No. : |
IBSP-2711H |
Product Overview : |
Recombinant Human Bone Sialoprotein (Phe17Gln317) fused a His-tag at C-terminal was expressed in CHO cells. |
Description : |
Bone sialoprotein (BSP) is a component of mineralized tissues such as bone, dentin, cementum and calcified cartilage. BSP is a significant component of the bone extracellular matrix and has been suggested to constitute approximately 8% of all non-collagenous proteins found in bone and cementum. |
Source : |
CHO Cells |
Species : |
Human |
Predicted NTerminal : |
Phe17 |
SDS-PAGE : |
7590 kDa, reducing conditions |
Activity : |
Measured by the ability of the immobilized protein to support the adhesion of the MCF-7human breast cancer cells. When 5 x104 cells/well are added to recombinant human IBSP- coated plates (3 µg/mL with 100 µL/well), approximately 60-80% will l adhereafter 30 minutes at 37°C. |
Endotoxin : |
Less than 1.0 EU per 1 μg of the protein by the LAL method. |
Purity : |
>90%, by SDSPAGE under reducing conditions and visualized by silver stain. |
Formulation : |
Lyophilized from a 0.2 μm filtered solution in MES and NaCl. See Certificate of Analysis for details. |
Reconstitution : |
Reconstitute at 100 μg/mL in sterile PBS. |
Storage : |
12 months from date of receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditions after reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution. Avoid repeated freeze¬thaw cycles. |