| Species : |
Human |
| Source : |
Mammalian Cells |
| Tag : |
His |
| Description : |
The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Bio-activity : |
Measured by its binding ability in a functional ELISA. When Recombinant Human (rh) TLR4 is Immobilized at 2 μg/mL (100 μL/well), the concentration of rhMD2 that produces 50% optimal binding response is found to be approximately 0.03-0.15 μg/mL. |
| Molecular Mass : |
Predicted Molecular Weight: 70.6 kDa
SDS-PAGE: 90-95 kDa, reducing conditions |
| Endotoxin : |
<0.10 EU per 1 μg of the protein by the LAL method |
| Purity : |
>95% by SDS-PAGE |
| Storage : |
Store at -20 to -80 centigrade in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. |
| Reconstitution : |
Reconstitute at 100 μg/mL in PBS. |
