Recombinant Human CSF1 protein
Cat.No. : | CSF1-289H |
Product Overview : | Recombinant Human CSF1 protein was expressed in Escherichia coli. |
- Specification
- Gene Information
- Related Products
Description : | Macrophage Colony Stimulating Factor (M-CSF), also named CSF-1, is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It is produced by osteoblasts (as a result of endocrine stimulation by parathyroid hormone) exerts paracrine effects on osteoclasts and can interact with CSF1R. M-CSF is a four α-helical bundle cytokine and its active form is found extracellularly as a disulfide-linked homodimer. Four transcript variants encoding three different isoforms have been reported for M-CSF gene. Although forms may vary, all of them contain the N-terminal 150 a.a. portion that is necessary and sufficient for interaction with the receptor. The first 223 a.a. of mature human M-CSF shares 88 %, 86 %, 81 % and 74 % sequence identity with corresponding regions of dog, cow, mouse and rat M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific. |
Source : | E.coli |
Species : | Human |
Form : | Lyophilized from a 0.2μm filtered solution in PBS, pH7.4. |
Bio-activity : | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine M-NFS-60 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁶ IU/mg. |
Molecular Mass : | Approximately 36.8 kDa, a disulfide-linked homodimer consisting of two 158 amino acid polypeptide chains. |
Protein length : | 158 |
AA Sequence : | EEVSEYCSHMIGSGHLQSLQRLIDS QMETSCQITFEFVDQEQLKDPVCYL KKAFLLVQDIMEDTMRFRDNTPNAI AIVQLQELSLRLKSCFTKDYEEHDK ACVRTFYETPLQLLEKVKNVFNETK NLLDKDWNIFSKNCNNSFAECSSQG HERQSEGS |
Endotoxin : | Less than 1 EU/μg of rHuM-CSF as determined by LAL method. |
Purity : | >95% by SDS-PAGE and HPLC analysis. |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
Reconstitution : | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water to a concentration of 0.1 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
Gene Name : | CSF1 |
Official Symbol : | CSF1 |
Synonyms : | CSF1; colony stimulating factor 1 (macrophage); macrophage colony-stimulating factor 1; M CSF; MCSF; MGC31930; lanimostim; CSF-1; |
Gene ID : | 1435 |
mRNA Refseq : | NM_000757 |
Protein Refseq : | NP_000748 |
MIM : | 120420 |
UniProt ID : | P09603-3 |
Products Types
◆ Recombinant Protein | ||
CSF1-41H | Active Recombinant Human CSF1 Protein, Animal Free | +Inquiry |
CSF1-45H | Recombinant Active Human CSF1 Protein, His-tagged(C-ter) | +Inquiry |
Csf1-041M | Active Recombinant Mouse Csf1 Protein | +Inquiry |
CSF1-4398B | Recombinant Bovine CSF1 Protein | +Inquiry |
Csf1-334C | Active Recombinant Mouse Csf1 Protein (156 aa) | +Inquiry |
◆ Lysates | ||
CSF1-1934HCL | Recombinant Human CSF1 cell lysate | +Inquiry |
CSF1-001MCL | Recombinant Mouse CSF1 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (5)
Ask a questionCSF1 is implicated in tumor-associated macrophages, influencing tumor growth, invasion, and the tumor microenvironment.
Side effects may include immune-related adverse events, inflammation, and alterations in white blood cell counts.
Yes, there is ongoing research on combining CSF1-targeted therapies with other immunotherapies or conventional treatments to enhance efficacy.
Challenges include maintaining the balance of immune responses, potential off-target effects, and variability in patient responses.
Studies are exploring its role in neuroinflammation, neuroprotection, and potential therapeutic interventions for conditions like Alzheimer's disease.
Customer Reviews (3)
Write a reviewThe CSF1 protein is renowned for its exceptional quality and reliability, making it an ideal choice to fulfill my experimental requirements.
With its high purity and stability, this protein ensures dependable and accurate results, which are crucial for the success of my research endeavors.
Whether it is for investigating cellular pathways, studying protein functions, or exploring signaling cascades, the CSF1 protein offers unparalleled versatility and performance.
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