"CSF1" Related Products

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Recombinant Human Colony Stimulating Factor 1 (Macrophage)

Cat. No.: CSF1-289H
Product Overview: Recombinant Human M-CSF produced in E.coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 159 amino acids and having a total molecular mass of 36.8 KDa.
Description: Macrophage colony-stimulating factor, or M-CSF, is a secreted cytokine which influences hematopoietic stem cells to differentiate into macrophages or other related cell types.
Species: Human
Source: E.coli
Form: Human MCSF is lyophilized in 10 mM NaP, 8.0 + 50 mM NaCl, at a concentration of 1 mg/ml.
Purity: Purity Greater than 97% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Bio-activity: Recombinant Human Macrophage Colony Stimulating Factor is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of mouse NFS-60 cells is <2.0 ng/ml.
Physical Appearance: Sterile Filtered white lyophilized (freeze-dried) powder.
Solubility: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mgμs/ml, which can be further diluted into other aqueous solutions.
Purity Greater than 97% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Protein Content determined by: UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Endotoxin Level: Endotoxin level is <0.01ng/ug or <0.1EU/ug.
Amino acid sequence: MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS
Storage: Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Gene Name: CSF1 colony stimulating factor 1 (macrophage) [ Homo sapiens ]
Official Symbol: CSF1
Synonyms: CSF1; colony stimulating factor 1 (macrophage); MCSF; MGC31930; colony stimulating factor 1; OTTHUMP00000013363; OTTHUMP00000013364; macrophage colony stimulating factor; M-CSF; CSF-1; Lanimostim; OTTHUMP00000013362
Gene ID: 1435
mRNA Refseq: NM_000757
Protein Refseq: NP_000748
MIM: 120420
UniProt ID: P09603
Chromosome Location: 1p21-p13
Pathway: Cytokine-cytokine receptor interaction; Hematopoietic cell lineage
Function: cytokine activity; growth factor activity; macrophage colony stimulating factor receptor binding; protein binding; protein homodimerization activity

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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