"Ifng" Related Products

Recombinant Mouse Interferon Gamma

Cat. No. : Ifng-114M
Product Overview : Recombinant Murine IFN-gamma produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 134 amino acids and having a molecular mass of 15,601 Daltons.
Description : The major interferon produced by mitogenically or antigenically stimulated lymphocytes. It is structurally different from type I interferon and its major activity is immunoregulation. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. The synthesis of IFN-gamma is induced, among other things, by Interleukin-2, FGF-basic, and EGF.
Species : Mouse
Source : E.coli
Form : Murine IFN-g was lyophilized from 0.5X PBS pH-7.2.
Purity : Purity Greater than 95% as determined by: Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Bio-activity : Recombianant Mouse Interferon gamma is fully biologically active when compared to standard. The specific activity as determined in a viral challenge assay using EMC virus on L929 cells is 1.3 x 107 Units/mg. The corresponding ED50 is 0.61ng/ml.
Physical Appearance : Sterile Filtered white lyophilized (freeze-dried) powder.
Solubility : Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mgμs/ml, which can be further diluted into other aqueous solutions.
Purity Greater than 95% as determined by : Analysis by RP-HPLC. Reducing and non-reducing SDS-PAGE.
Protein Content determined by : UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard.
Endotoxin Level : Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
Storage : Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage.
Pathways : Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Natural killer cell mediated cytotoxicity; Regulation of autophagy; T cell receptor signaling pathway; TGF-beta signaling pathway; Type I diabetes mellitus
Gene Name : Ifng interferon gamma [ Mus musculus ]
Official Symbol : Ifng
Synonyms : Ifng; IFN-g; IFN-gamma; Immune Interferon; type II interferon; T cell interferon; MAF; IFNG; IFG; IFI; gamma interferon; Ifg; interferon gamma
Gene ID : 15978
mRNA Refseq : NM_008337
Protein Refseq : NP_032363
UniProt ID : P01580
Chromosome Location : 10 D2; 10 67.0 cM
Function : cytokine activity; interferon-gamma receptor binding

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