Active Recombinant Human Interleukin 10
Cat.No. : | IL10-478H |
Product Overview : | Interleukin-10 Human Recombinant produced in e.coli is a single, non-glycosylated polypeptide chain containing 161 amino acids and having a molecular mass of 16.6 kDa. |
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Cat. No. : | IL10-478H |
Description : | Interleukin-10 is produced by murine T-cells following their stimulation by lectins. The main source for B-cell-derived Interleukin-10 in mice are Ly-1 B-cells that express CD5 and CD11. Murine keratinocytes also produce IL10. |
Species : | Human |
Source : | E.coli |
Form : | Lyopohilized from 10 mM NaP, pH 7.5. |
Purity : | Purity Approximately 90% as determined by: Reducing and non-reducing SDS-PAGE. |
Bio-activity : | Interleukin-10 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of mouse MC-9 cells was found to be < 0.5 ng/ml, corresponding to a Specific Activity of 3 x 10⁶ IU/mg. |
Physical Appearance : | Sterile Filtered white lyophilized (freeze-dried) powder. |
Solubility : | Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg"s/ml, which can be further diluted into other aqueous solutions. |
Purity Greater than 90% as determined by : | Reducing and non-reducing SDS-PAGE. |
Protein Content determined by : | UV spectroscopy at 280 nm. Analysis by RP-HPLC calibrated against a known standard. Quantitation on SDS-PAGE against a known standard. |
Endotoxin Level : | Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug. |
Amino acid sequence : | MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI |
Storage : | Lyophilized product is very stable at -20 degrees. Reconstituted material should be aliquoted and frozen at -20. It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage. |
Pathways : | Allograft rejection; Asthma; Autoimmune thyroid disease; Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Systemic lupus erythematosus; T cell receptor signaling pathway |
Gene Name : | IL10 interleukin 10 [ Homo sapiens ] |
Official Symbol : | IL10 |
Synonyms : | IL10; CSIF; TGIF; IL-10; IL10A; MGC126450; MGC126451; IL10; interleukin 10; cytokine synthesis inhibitory factor; Interleukin-10; Cytokine synthesis inhibitory factor |
Gene ID : | 3586 |
mRNA Refseq : | NM_000572 |
Protein Refseq : | NP_000563 |
MIM : | 124092 |
UniProt ID : | P22301 |
Chromosome Location : | 1q31-q32 |
Function : | cytokine activity; protein binding ; interleukin-10 receptor binding; growth factor activity |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionCutting-edge imaging techniques, alongside in vivo disease models, allow for precise assessment of therapeutic effects and tissue responses to IL-10-based treatments.
Advanced technologies, including single-cell RNA sequencing and flow cytometry, enable a granular analysis of IL-10's impact on diverse immune cell subpopulations.
Genotyping studies and genetic association analyses help correlate variations in IL-10 and its receptor with disease susceptibility across diverse populations.
Advanced immunomonitoring tools, including multiplex cytokine assays and immune cell phenotyping, provide comprehensive safety evaluations of IL-10 interventions in clinical trials.
In vitro models involve exposing immune cells to IL-10 and assessing changes in cytokine secretion and cell activation markers to uncover its anti-inflammatory mechanisms.
State-of-the-art techniques such as phosphoproteomics, mass spectrometry, and protein-protein interaction assays provide insights into the intricate signaling cascades activated by IL-10.
Dose-response studies in animal models coupled with outcome measures like cytokine profiles and immune cell phenotypes help determine the optimal IL-10 dosage and administration routes.
Experimental designs encompass co-stimulation experiments, coupled with functional assays and high-dimensional analysis, to elucidate synergistic effects involving IL-10 and other cytokines.
Techniques like RT-PCR, immunohistochemistry, and spatial transcriptomics are employed to characterize IL-10 receptor expression patterns in specific tissues and disease contexts.
CRISPR/Cas9 gene editing is used to create knockout or overexpression models, combined with functional assays, to decipher IL-10's regulatory roles in autoimmune disease pathways.
Customer Reviews (3)
Write a reviewEffective in anti-inflammatory assays.
High purity levels.
Stable under long-term storage.
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