Recombinant Human PRLP, GST-tagged

• Cat.No.: PRLP-3704H
• Product Overview: Cytoplasmatic PRLP Human Recombinant produced in E.coli is a non-glycosylated, Polypeptide chain amino acids 432-623 and having a molecular mass of 45 kDa, the PRLR is fused with a GST tag. PRLP is purified by proprietary chromatographic techniques.

Specification

• Cat. No.: PRLP-3704H
• Description: PRLP encoded by a gene on Chromosome 5p13-14 interacts with the prolactin molecule as a transmembrane receptor. Thus it contains an extracellular region that binds prolactin, a transmembrane regions, and a cytoplasmatic region. The receptor is a cytokine receptor, and the second messenger cascade is the JAK-STAT pathway. There may be variations among different tissue PRLP.
• Source: E. coli
• Species: Human
• Form: PRLR Human at 0.1 mg/ml in 50 mM Tris-Acetate, pH 7.5, 1 mM EDTA and 20% Glycerol.
• Research topic: Energy metabolism and body weight regulation
• Applications: ELISA; Inhibition Assays; Western blotting
• Storage: Store vial at -20°C to -80°C. When stored at the recommended temperature, this protein is stable for 12 months. Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: PRLR prolactin receptor [ Homo sapiens ]
• Official Symbol: PRLR
• Synonyms: PRLR; prolactin receptor; hPRLrI; PRL-R; hPRL receptor; OTTHUMP00000115998; OTTHUMP00000221070; OTTHUMP00000221071; OTTHUMP00000221073; secreted prolactin binding protein; delta 4-SF1b truncated prolactin receptor; delta 4-delta 7/11 truncated prolactin receptor
• Gene ID: 5618
• mRNA Refseq: NM_000949
• Protein Refseq: NP_000940
• MIM: 176761
• UniProt ID: P16471
• Chromosome Location: 5p14-p13
• Pathway: Adipogenesis; Cytokine-cytokine receptor interaction; ErbB4 signaling events; Jak-STAT signaling pathway; Neuroactive ligand-receptor interaction; Ovarian Infertility Genes; Signaling events mediated by PTP1B
• Function: cytokine receptor activity; metal ion binding; ornithine decarboxylase activator activity; peptide hormone binding; prolactin receptor activity; protein binding; protein homodimerization activity; receptor activity

Reviews

04/03/2022

Consistent quality shines through—this protein has become indispensable.

02/24/2021

This protein streamlines our experiments.

12/22/2018

Fast shipping made this purchase convenient for our lab operations.

Q&As

How does PRLR contribute to the development of breast cancer at the molecular level?

PRLR overexpression or dysregulation has been associated with breast cancer. It can promote cell proliferation and survival through aberrant signaling pathways.

How does PRLR signaling vary in different tissues?

Tissue-specific factors and the presence of co-receptors contribute to the diversity of PRLR signaling outcomes in different tissues and cell types.

What are the key signaling pathways activated by the PRLR protein?

The JAK-STAT signaling pathway is a primary pathway activated by PRLR. It involves the activation of Janus kinases (JAKs) and signal transducers and activators of transcription (STATs).

Explain the process of endocytosis and intracellular trafficking of PRLR.

PRLR undergoes endocytosis upon ligand binding, and the internalized receptor-ligand complex is trafficked through intracellular compartments, influencing downstream signaling and receptor recycling.

Discuss the post-translational modifications involved in regulating PRLR activity.

Phosphorylation is a crucial post-translational modification of PRLR, affecting its activation and downstream signaling. Other modifications, such as glycosylation, also play roles.