Recombinant Human PRLR protein, His-tagged

• Cat.No.: PRLR-177H
• Product Overview: Recombinant Human PRLR protein(P16471-1)(Gln25-Asp234), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: Gln25-Asp234
• Tag: C-His
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human PRLR, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PRLR Antibody, hFc Tag with the EC50 of 28.2ng/ml determined by ELISA (QC Test).
• Molecular Mass: The protein has a predicted MW of 25.5 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PRLR prolactin receptor [ Homo sapiens ]
• Official Symbol: PRLR
• Synonyms: PRLR; prolactin receptor; PRL-R; hPRL receptor; secreted prolactin binding protein; hPRLrI
• Gene ID: 5618
• mRNA Refseq: NM_000949
• Protein Refseq: NP_000940
• MIM: 176761
• UniProt ID: P16471