Active Recombinant Human PRLR protein, His-tagged, Low Endotoxin

• Cat.No.: PRLR-177H
• Product Overview: Recombinant Human PRLR protein(P16471-1)(Gln25-Asp234), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 25-234 aa
• Form: Lyophilized from 0.22μm filtered solution in 50mM Tris, 150nM Nacl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human PRLR, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-PRLR Antibody, hFc Tag with the EC50 of 28.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 25.5 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 24 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: PRLR prolactin receptor [ Homo sapiens ]
• Official Symbol: PRLR
• Synonyms: PRLR; prolactin receptor; PRL-R; hPRL receptor; secreted prolactin binding protein; hPRLrI
• Gene ID: 5618
• mRNA Refseq: NM_000949
• Protein Refseq: NP_000940
• MIM: 176761
• UniProt ID: P16471