Recombinant Human Protein Kinase D2

• Cat.No.: PRKD2-1130H
• Product Overview: Recombinant human PRKD2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Description: PRKD2, also known as PRKD2 and PKD2, is a cytoplasm and membrane protein which belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and PKD subfamily. PRKD2 / PKD2 is widely expressed. It contains one PH domain, two phorbol-ester/DAG-type zinc fingers and one protein kinase domain. PRKD2 / PKD2 is activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains bind DAG, mediating translocation to membranes. Autophosphorylation of Ser-710 and phosphorylation of Ser-706 by PKC relieves auto-inhibition by the PH domain. PRKD2 / PKD2 converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
• Biological Activity: 259 nmol phosphate incorporated into CREBtide substrate peptide per minute per mg protein at 30°C for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).
• Molecular Weight: 130.0 kDa
• Form: Recombinant proteins in storage buffer (50 mMTris-HCl, pH 7.5, 150 mMNaCl, 0.25 mM DTT, 0.1 MmEGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol).
• Appearance: Liquid
• Purity: ≥80% by SDS-PAGE
• Storage: Store product frozen at or below -70°C. Stable for 1 year at -70°C as undiluted stock. Aliquot to avoidrepeated thawing and freezing.
• Pathways: T Cell Receptor Signaling Pathway

Gene Information

• Gene Name: PRKD2 protein kinase D2 [ Homo sapiens ]
• Official Symbol: PRKD2
• Synonyms: PRKD2; protein kinase D2; PKD2; HSPC187; serine/threonine-protein kinase D2; nPKC-D2; EC 2.7.11.13; DKFZP586E0820
• Gene ID: 25865
• mRNA Refseq: NM_001079880
• Protein Refseq: NP_001073349
• MIM: 607074
• UniProt ID: Q9BZL6
• Chromosome Location: 19q13.3
• Function: ATP binding; metal ion binding; nucleotide binding; protein kinase C activity; protein serine/threonine kinase activity; transferase activity