Recombinant Human PRKD2 protein(Met1-Leu878), His&GST-tagged

• Cat.No.: PRKD2-763H
• Product Overview: Recombinant Human PRKD2 (NP_057541.2)(Met 1-Leu 878) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST&His
• Protein Length: 1-878 a.a.
• Form: Supplied as sterile 50mM Tris, 500mM NaCl, 0.5mM PMSF, 10% glycerol, pH 8.0
• Bio-activity: The specific activity was determined to be ＞30 nmol/min/mg using synthetic CREBtide peptide (KRREILSRRPSYR) as substrate.
• Molecular Mass: The recombinant human PRKD2/GST chimera consists of 1115 amino acids and has a calculated molecular mass of 124 kDa. It migrates as an approximately 120 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 82 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: PRKD2 protein kinase D2 [ Homo sapiens ]
• Official Symbol: PRKD2
• Synonyms: PRKD2; protein kinase D2; serine/threonine-protein kinase D2; DKFZP586E0820; HSPC187; PKD2; nPKC-D2
• Gene ID: 25865
• mRNA Refseq: NM_001079880
• Protein Refseq: NP_001073349
• MIM: 607074
• UniProt ID: Q9BZL6