Recombinant Human Histone Deacetylase 8

• Cat.No.: HDAC8-1655H

Specification

• Cat. No.: HDAC8-1655H
• Description: HDAC8 is a member of the class I Histone Deacetylases. HDAC8 are important enzymes for the transcriptional regulation of gene expression in eukaryotic cells. HDAC8 catalyze the removal of acetyl groups from lysines near the N-termini of histones. Human HDAC8 have been implicated in a variety of human diseases such as cardiomyopathy, osteodystrophy, neurodegenerative disorders, aging and cancer. Expression of HDAC8 is restricted to cells showing smooth muscle differentiation in normal human tissue and is a novel marker of smooth muscle differentiation. Like other class I and II HDAC members, the activity of HDAC8 is sensitive to HDAC inhibitor Trichostatin A (TSA).
• Biological Activity: Activity of rh-HDAC8 was tested using Arg-His-Lys (Ac)-Ly (Ac)-AMC, a fluorogenic, acetylated peptide based on residues 379-382 of p53 as a substrate.
• Molecular Weight: 44 kDa
• Source: Sf9 insect cells
• Species: Human
• Form: Liquid in 25 mM Tris, pH 7.5, 100 mM NaCl, 2.7 mM KCl, 3 mM MgCl2, 10 % glycerol.
• Appearance: Liquid
• Purity: > 90% by SDS-PAGE
• Storage: Stable for 1 year at -70°C. Avoid multipie freeze/thaw cycles as activity may decrease.
• Pathways: Cell cycle; Signaling events mediated by HDAC Class I

Gene Information

• Gene Name: HDAC8 histone deacetylase 8 [ Homo sapiens ]
• Official Symbol: HDAC8
• Synonyms: HDAC8; histone deacetylase 8; RPD3; HDACL1; histone deacetylase-like 1; EC 3.5.1.98; HD8
• Gene ID: 55869
• mRNA Refseq: NM_018486
• Protein Refseq: NP_060956
• MIM: 300269
• UniProt ID: Q9BY41
• Chromosome Location: Xq13
• Function: histone deacetylase activity; hydrolase activity; transcription factor binding; metal ion binding; histone deacetylase activity