Recombinant Human Heat Shock 27kDa Protein 1, GST-tagged


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Recombinant Human Heat Shock 27kDa Protein 1, GST-tagged

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Cat. No. : HSPB1-4853H
Product Overview : Recombinant full-length human HSPB1 was expressed in E.coli cells using a N-terminal GST tag.
Description : HSPB1 is a member of heat shock proteins (HSPs) which are synthesized in cells in response to heat shock and other metabolic stresses and provide a transient state of thermotolerance. HSPB1 plays a major role in the increased thermal resistance acquired by cells after exposure to HSP inducers. The level of HSPB1 phosphorylation is significantly elevated after exposure of cells to heat shock, sodium arsenite, IL-1 and TNF. MAPKAPK2 and MAPKAPK3 are both activated by these conditions and can phosphorylate HSPB1 on serine residues.
Applications : Kinase Assay; Western Blot
Molecular Weight : 52 kDa
Expression System : E.coli
Species : Human
Form : Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 0,25 mM DTT, 0.1 mM PMSF, 25% glycerol.
Purity : > 80 %
Concentration : 0.2 ug/ul
Sequences : Full-lengt h
Storage : Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Pathways : Amoebiasis; Destabilization of mRNA by AUF1 (hnRNP D0); FAS pathway and Stress induction of HSP regulation; IL-3 Signaling Pathway; IL-6 Signaling Pathway; MAPK signaling pathway; Metabolism of RNA; Metabolism of mRNA; Regulation of mRNA Stability by Proteins that Bind AU-rich Elements; Signaling events mediated by VEGFR1 and VEGFR2; Signaling mediated by p38-alpha and p38-beta; TNF-alpha/NF-kB Signaling Pathway; VEGF signaling pathway; p38 MAPK Signaling Pathway; p38 signaling mediated by MAPKAP kinases
Gene Name : HSPB1 heat shock 27kDa protein 1 [ Homo sapiens ]
Official Symbol : HSPB1
Synonyms : HSPB1; heat shock 27kDa protein 1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; DKFZp586P1322; HS.76067; HSPB1; OTTHUMP00000024846; heat shock protein beta-1; HSPB1 heat shock 27kDa protein 1; 28 kDa heat shock protein; Estrogen-regulated 24 kDa protein; Heat shock 27 kDa protein; Stress-responsive protein 27; heat shock 27kD protein 1; heat shock 27kDa protein 1; heat shock protein beta-1
Gene ID : 3315
mRNA Refseq : NM_001540
Protein Refseq : NP_001531
MIM : 602195
UniProt ID : P04792
Chromosome Location : 7q11.23
Function : protein binding; protein kinase C delta binding; protein kinase C inhibitor activity; ubiquitin binding

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