Cat. No.: |
MELK-1059H |
Product Overview: |
Recombinant human MELK (1-340) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. |
Description: |
MELK or maternal embryonic leucine zipper kinase is a member of the CAMKL kinase family. MELK is a key regulator of the proliferation of malignant brain tumors including their stem cells (1). MELK transcript abundance correlates with malignancy grade in human astrocytomas and represents a therapeutic target for the management of the most frequent brain tumors in adult and children. MELK also plays a role in mammary carcinogenesis through inhibition of the pro-apoptotic function of Bcl-GL (2). Therefore, the kinase activity of MELK could be a promising molecular target for development of therapy for patients with breast cancers. |
Applications: |
Kinase Assay; Western Blot |
Molecular Weight: |
61 kDa |
Expression System: |
Sf9 insect cells using baculovirus |
Species: |
Human |
Form: |
Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol |
Specific Activity: |
200 nmol/min/mg |
Purity: |
> 85% |
Concentration: |
0.1 ug/ul |
Sequences: |
1-340 |
Storage: |
Store product at -70oC. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. |