Species : |
Mouse |
Source : |
Mouse |
Tag : |
His |
Protein Length : |
1-291 a.a. |
Description : |
Car8 are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. Car8 participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. |
Form : |
Lyophilized from sterile 50 mM Tris, pH 8.0. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
Purity : |
> 88 % as determined by SDS-PAGE |
Bio-activity : |
Measured by its esterase activity. The specific activity is > 5 pmoles/min/μg. |
Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method. |
Stability : |
Samples are stable for up to twelve months from date of receipt at -70°C. |
Predicted N terminal : |
Met 1 |
Molecular Mass : |
The recombinant mouse Car8 comprises 301 amino acids and has a calculated molecular mass of 34.5 kDa. The recombinant protein migrates as an approximately 37 kDa band in SDS-PAGE under reducing condition. |
Application : |
SDS-PAGE |
Reconstitution : |
A hardcopy of COA with reconstitution instruction is sent along with the products. |
Storage : |
Store it under sterile conditions at -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Pathways : |
Nitrogen metabolism |