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| Cat. No.: | Car8-1804M |
| Product Overview: | A DNA sequence encoding the mouse Car8 (P28651)( Met 1-Gln 291) was expressed, with a C-terminal polyhistidine tag. |
| Description: | Car8 are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. Car8 participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. |
| Source: | Mouse |
| Host: | E.coli |
| Form: | Lyophilized from sterile 50 mM Tris, pH 8.0. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Purity: | > 88 % as determined by SDS-PAGE |
| Bio-activity: | Measured by its esterase activity. The specific activity is > 5 pmoles/min/μg. |
| Endotoxin: | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Stability: | Samples are stable for up to twelve months from date of receipt at -70°C. |
| Predicted N terminal: | Met 1 |
| Molecular Mass: | The recombinant mouse Car8 comprises 301 amino acids and has a calculated molecular mass of 34.5 kDa. The recombinant protein migrates as an approximately 37 kDa band in SDS-PAGE under reducing condition. |
| Application: | SDS-PAGE |
| Reconstitution: | A hardcopy of COA with reconstitution instruction is sent along with the products. |
| Storage: | Store it under sterile conditions at -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Pathways: | Nitrogen metabolism |
| Gene Name: | Car8 carbonic anhydrase 8 [ Mus musculus ] |
| Official Symbol: | Car8 |
| Synonyms: | Car8; carbonic anhydrase 8; Ca8; wdl; Cals; Carp; Cals1; AW546993; carbonic anhydrase-related protein; CA-VIII; CA-RP VIII; OTTMUSP00000004709; carbonic anhydrase VIII; carbonic anhydrase-like sequence 1; NP_031618.2; EC 4.2.1.1 |
| Gene ID: | 12319 |
| mRNA Refseq: | NM_007592 |
| Protein Refseq: | NP_031618 |
| UniProt ID: | P28651 |
| Chromosome Location: | 4 |
| Function: | carbonate dehydratase activity; metal ion binding; protein binding; zinc ion binding |
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