"CA8" Related Products

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Recombinant Mouse Carbonic Anhydrase 8, His-tagged

Cat. No.: Car8-1804M
Product Overview: A DNA sequence encoding the mouse Car8 (P28651)( Met 1-Gln 291) was expressed, with a C-terminal polyhistidine tag.
Description: Car8 are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. Car8 participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid.
Source: Mouse
Host: E.coli
Form: Lyophilized from sterile 50 mM Tris, pH 8.0. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Purity: > 88 % as determined by SDS-PAGE
Bio-activity: Measured by its esterase activity. The specific activity is > 5 pmoles/min/μg.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Stability: Samples are stable for up to twelve months from date of receipt at -70°C.
Predicted N terminal: Met 1
Molecular Mass: The recombinant mouse Car8 comprises 301 amino acids and has a calculated molecular mass of 34.5 kDa. The recombinant protein migrates as an approximately 37 kDa band in SDS-PAGE under reducing condition.
Application: SDS-PAGE
Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products.
Storage: Store it under sterile conditions at -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Pathways: Nitrogen metabolism
Gene Name: Car8 carbonic anhydrase 8 [ Mus musculus ]
Official Symbol: Car8
Synonyms: Car8; carbonic anhydrase 8; Ca8; wdl; Cals; Carp; Cals1; AW546993; carbonic anhydrase-related protein; CA-VIII; CA-RP VIII; OTTMUSP00000004709; carbonic anhydrase VIII; carbonic anhydrase-like sequence 1; NP_031618.2; EC 4.2.1.1
Gene ID: 12319
mRNA Refseq: NM_007592
Protein Refseq: NP_031618
UniProt ID: P28651
Chromosome Location: 4
Function: carbonate dehydratase activity; metal ion binding; protein binding; zinc ion binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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