Recombinant Human CA8 Protein, MYC/DDK-tagged

• Cat.No.: CA8-2273H
• Product Overview: Recombinant human CA8 protein, fused to MYC/DDK-tagged at C-terminus, was expressed in HEK293

Specification

• Description: The protein encoded by this gene was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, the gene product lacks carbonic anhydrase activity (i.e., the reversible hydration of carbon dioxide). The gene product continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. The absence of CA8 gene transcription in the cerebellum of the lurcher mutant in mice with a neurologic defect suggests an important role for this acatalytic form. Mutations in this gene are associated with cerebellar ataxia, mental retardation, and dysequilibrium syndrome 3 (CMARQ3). Polymorphisms in this gene are associated with osteoporosis, and overexpression of this gene in osteosarcoma cells suggests an oncogenic role. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016].
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Form: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 32.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: CA8 carbonic anhydrase VIII [ Homo sapiens ]
• Official Symbol: CA8
• Synonyms: CA-RP; CA-VIII; CALS; CAMRQ3; CARP
• Gene ID: 767
• mRNA Refseq: NM_004056
• Protein Refseq: NP_004047
• MIM: 114815
• UniProt ID: P35219

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Q&As

Are there specific proteins or molecules known to interact with CA8?

CA8 interacts with IP3Rs, affecting calcium signaling in neurons.

How does CA8's role differ from the enzymatically active carbonic anhydrase isoforms?

Unlike enzymatically active isoforms, CA8 doesn't catalyze the hydration of CO2 but instead plays roles in neuronal signaling and function.

Are there genetic mutations in the CA8 gene that are linked to specific diseases?

Mutations in CA8 have been linked to cerebellar ataxia, a movement disorder.

How does CA8 contribute to cellular processes, given its non-enzymatic nature?

CA8 impacts calcium signaling in neurons, affecting neural communication and potentially influencing motor control.

What post-translational modifications are typical for CA8?

Specific modifications, like phosphorylation, can be explored using mass spectrometry or targeted biochemical assays.

What is the primary function of CA8, considering it's a non-enzymatic member of the carbonic anhydrase family?

CA8 acts as a stabilizer for inositol 1,4,5-trisphosphate receptors (IP3Rs) in neurons.

In which human tissues or cell types is CA8 predominantly expressed?

CA8 is mainly expressed in the cerebellum, particularly in Purkinje cells.