Active Native Bovine IAP

• Cat.No.: IAP-8323C
• Product Overview: The Alkaline Phosphatase is purified by affinity chromatography, which results in an enzyme of high specific activity and purity.

Specification

• Description: Alkaline Phosphatase is Dimeric protein having molecular weight of 140 kDa, one Zn++ ion is tightly bound to each subunit, and another less tightly bound is involved in the catalytic reaction. Mg++ stimulates the catalysis. The binding site for Mg++ is different to that of Zn++, but will be occupied by excess Zn++ followed by loss of enzyme activity.
• Source: Calf
• Species: Calf
• Form: 50% glycerol, 5mM MgCl2, 0.1mM ZnCl2 and 5mM TRIS, pH 7.0.
• Bio-activity: >1000 U/mg (pH 9.6, 25C and 0.025M glycin, 10% glycerol as buffer.
• Purity: 95% pure by Gel Filtration.
• Storage: AP should be stored at 4 °C. Please Do-Not freeze.

Gene Information

• Gene Name: ALPI alkaline phosphatase, intestinal [ Bos taurus ]
• Official Symbol: ALPI
• Synonyms: IAP; intestinal-type alkaline phosphatase; intestinal alkaline phosphatase V
• Gene ID: 280993
• mRNA Refseq: NM_173987
• Protein Refseq: NP_776412
• Chromosome Location: 2q45
• Pathway: Folate biosynthesis, organism-specific biosystem; Folate biosynthesis, conserved biosystem; tetrahydrofolate biosynthesis I mammals, organism-specific biosystem
• Function: alkaline phosphatase activity; magnesium ion binding; zinc ion binding