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| Cat. No.: | IL10-40H |
| Product Overview: | Recombinant Human Interleukin-10 produced inCHOis a single, glycosylated polypeptide chain containing 161 amino acids and having a molecular mass of 18606.29 Dalton. The IL-10 is purified by proprietary chromatographic techniques. |
| Description: | IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. |
| Source: | Chinese Hamster Ovary Cells |
| Amino Acid Sequence: | The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Gly-Gln-Thr. |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Purity: | Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Formulation: | The protein was lyophilized from a concentrated (1mg/ml) solution containing 60mM PBS pH-6.5, 50mg BSA and 100mM NaCl. |
| Solubility: | It is recommended to reconstitute the lyophilized Interleukin-10 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
| Biological Activity: | The ED50as determined by the dose-dependant co-stimulation with murine IL-4 of MC-9 cells was found to be < 2ng/ml, corresponding to a Specific Activity of 1.5 x 107 IU/mg. |
| Storage: | Lyophilized Interleukin 1 although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution IL10 should be stored at 4℃ between 2-7 days and for future use below -18℃. Please prevent freeze-thaw cycles. |
| Gene Name: | IL10 interleukin 10 [ Homo sapiens ] |
| Synonyms: | CSIF; TGIF; IL-10; IL10A; MGC126450; MGC126451; IL10; interleukin 10; cytokine synthesis inhibitory factor; Interleukin-10; Cytokine synthesis inhibitory factor |
| Gene ID: | 3586 |
| mRNA Refseq: | NM_000572 |
| Protein Refseq: | NP_000563 |
| MIM: | 124092 |
| UniProt ID: | P22301 |
| Chromosome Location: | 1q31-q32 |
| Pathway: | Allograft rejection; Asthma; Autoimmune thyroid disease; Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Systemic lupus erythematosus; T cell receptor signaling pathway |
| Function: | cytokine activity; protein binding ; interleukin-10 receptor binding; growth factor activity |
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