Species : |
Human |
Source : |
CHO |
Tag : |
Non |
Description : |
IL10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. |
Amino Acid Sequence : |
The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Gly-Gln-Thr. |
Physical Appearance : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity : |
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Formulation : |
The protein was lyophilized from a concentrated (1mg/ml) solution containing 60mM PBS pH-6.5, 50mg BSA and 100mM NaCl. |
Solubility : |
It is recommended to reconstitute the lyophilized Interleukin-10 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Biological Activity : |
The ED50as determined by the dose-dependant co-stimulation with murine IL-4 of MC-9 cells was found to be < 2ng/ml, corresponding to a Specific Activity of 1.5 x 10⁷ IU/mg. |
Storage : |
Lyophilized Interleukin 1 although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution IL10 should be stored at 4℃ between 2-7 days and for future use below -18℃. Please prevent freeze-thaw cycles. |